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紫外线和γ射线照射的噬菌体DNA中特定抗原决定簇的免疫识别

Immunological recognition of specific antigenic determinants in UV- and gamma-irradiated phage DNA.

作者信息

Hotz G

出版信息

Radiat Environ Biophys. 1975 Jun 13;12(1):41-50. doi: 10.1007/BF02339808.

DOI:10.1007/BF02339808
PMID:52163
Abstract

Thermally denatured DNA of coliphage T1 after treatment with uv-light (2537 A) and 60Co-gamma rays acts as a hapten with antigenic determinant groups specific for radiation-induced alterations of the macromolecule. After conjugation to methylated bovine serum albumin the DNA becomes immunogenic in rabbits. Antibodies against irradiated DNA do not react with unirradiated single-stranded DNA. Antigen-antibody complexes were demonstrated by CsCl-density gradient centrifugation. The decrease in buoyant density of the DNA is proportional to the amount of antibody protein bound to the antigen. By this means photoproducts as well as alterations due to ionizing radiation in DNA were detected independent of the type of antigen-antibody complex, i.e. precipitating or soluble aggregate.

摘要

用紫外线（2537埃）和60钴γ射线处理后的大肠杆菌噬菌体T1的热变性DNA，作为一种半抗原，带有对大分子辐射诱导改变具有特异性的抗原决定簇基团。与甲基化牛血清白蛋白结合后，DNA在兔体内具有免疫原性。抗辐照DNA的抗体不与未辐照的单链DNA反应。通过氯化铯密度梯度离心法证实了抗原-抗体复合物的存在。DNA浮力密度的降低与结合到抗原上的抗体蛋白量成正比。通过这种方法，检测到了DNA中的光产物以及由于电离辐射引起的改变，而与抗原-抗体复合物的类型无关，即沉淀型或可溶性聚集体。

相似文献

1
Immunological recognition of specific antigenic determinants in UV- and gamma-irradiated phage DNA.紫外线和γ射线照射的噬菌体DNA中特定抗原决定簇的免疫识别
Radiat Environ Biophys. 1975 Jun 13;12(1):41-50. doi: 10.1007/BF02339808.
2
Immunological reaction of UV-induced radiation damage in coliphage DNA.紫外线诱导的噬菌体DNA辐射损伤的免疫反应。
Z Naturforsch C Biosci. 1977 Jul-Aug;32(7-8):544-9. doi: 10.1515/znc-1977-7-811.
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Genomic integrity of T1 DNA after gamma-and ultraviolet irradiation.γ射线和紫外线照射后T1 DNA的基因组完整性
Biochim Biophys Acta. 1979 Feb 27;561(2):312-23. doi: 10.1016/0005-2787(79)90140-0.
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Evidence against clustering of thymine dimers in ultraviolet-irradiated DNA.关于紫外线照射的DNA中胸腺嘧啶二聚体不存在聚集现象的证据。
Nature. 1975 May 15;255(5505):245-7. doi: 10.1038/255245a0.
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Infectious DNA from coliphage TI. 3. The occurrence of single-strand breaks in stored, thermally-treated, and U.V.-irradiated molecules.来自大肠杆菌噬菌体TI的感染性DNA。3. 储存、热处理和紫外线照射的分子中单链断裂的出现情况。
Int J Radiat Biol Relat Stud Phys Chem Med. 1971;19(6):519-36. doi: 10.1080/09553007114550711.
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Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light.
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Analysis of irradiated material.
Res Prog Org Biol Med Chem. 1972;3 Pt 1:71-99.
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Specificity of antibodies to the modified DNA of phages SPO1 and T4.
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Cesium chloride density gradient study of some modifications induced by ultraviolet radiation on the denatured DNA molecule of phage T2.氯化铯密度梯度法研究紫外线对噬菌体T2变性DNA分子的某些诱导修饰作用。
Biophys J. 1969 Dec;9(12):1451-63. doi: 10.1016/S0006-3495(69)86465-9.
10
In vitro repair of UV-or x-irradiated bacteriophage T4 DNA by extract from blue-green alga Anacystis nidulans.利用蓝藻集胞藻提取物对紫外线或X射线照射的噬菌体T4 DNA进行体外修复
Mol Biol Rep. 1975 Jul;2(2):89-94. doi: 10.1007/BF00357537.

引用本文的文献

1
On the immunological detection of X-ray induced DNA damage.关于X射线诱导的DNA损伤的免疫学检测
Radiat Environ Biophys. 1981;19(4):259-64. doi: 10.1007/BF01324091.
2
Characteristics of excision repair of pyrimidine dimers in eukaryotic cells as assayed with anti-dimer sera.用抗二聚体血清检测真核细胞中嘧啶二聚体切除修复的特征。
Nucleic Acids Res. 1978 Nov;5(11):4273-81. doi: 10.1093/nar/5.11.4273.

本文引用的文献

1
PRODUCTION OF ANTIBODIES TO DENATURED DEOXYRIBONUCLEIC ACID (DNA).抗变性脱氧核糖核酸（DNA）抗体的产生
Proc Natl Acad Sci U S A. 1964 Aug;52(2):279-85. doi: 10.1073/pnas.52.2.279.
2
RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.大肠杆菌K-12中DNA上紫外线诱导胸腺嘧啶二聚体的释放
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3
THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.胸腺嘧啶二聚体从DNA中的消失：一种纠错机制。
Proc Natl Acad Sci U S A. 1964 Feb;51(2):226-31. doi: 10.1073/pnas.51.2.226.
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Fractionation of nucleic acids with the methylated albumin column.用甲基化白蛋白柱对核酸进行分级分离。
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5
Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light.
Science. 1966 Sep 30;153(3744):1666-7. doi: 10.1126/science.153.3744.1666.
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DNA repair mechanisms and their relation to mutation and recombination.DNA修复机制及其与突变和重组的关系。
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Infectious DNA from coliphage T1. I. Some properties of the spheroplast assay system.来自大肠杆菌噬菌体T1的感染性DNA。I.原生质球检测系统的一些特性。
Mol Gen Genet. 1969;104(2):178-94. doi: 10.1007/BF00272800.
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Recognition of nucleotide sequences.核苷酸序列的识别
Annu Rev Biochem. 1969;38:841-80. doi: 10.1146/annurev.bi.38.070169.004205.
9
Serologic estimation of thymine dimers in the deoxyribonucleic acid of bacterial and mammalian cells following irradiation with ultraviolet light and postirradiation repair.
J Biol Chem. 1972 Sep 25;247(18):5709-15.