Wang Zirui, Li Shuyao, Zhou Qiubai, Zhang Jinhua, Li Yongan, Li Youjie, Yuan Zhiwen, Huang Guanghua
College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang, 330045, China.
Anim Nutr. 2023 Mar 2;13:229-239. doi: 10.1016/j.aninu.2023.02.007. eCollection 2023 Jun.
The aim of this study was to examine the effects of dietary protein and lipid levels on the growth performance and homeostasis of the intestinal flora in . An 8-wk 3 × 3 two-factorial experiment was conducted to investigate the interaction between dietary crude protein (CP: 30%, 35%, 40%) and ether extract (EE: 6%, 10%, 14%) on the growth rate and the intestinal microflora of . A total of 2,160 fish (5.19 ± 0.01 g) were randomly allotted to 36 aquariums each with 60 fish. Fish were fed the experimental diet twice daily. Results revealed that weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio and net protein utilization significantly increased when increasing protein levels from 30% to 40% ( < 0.05). Both WGR and SGR enhanced first but reduced thereafter with maximum value at 10% lipid level as dietary lipid increased from 6% to 14% ( < 0.05). Significant interactions between protein and lipid were found with feed conversion rate, lipid efficiency ratio and net lipid utilization ( < 0.05). At the phylum level, Proteobacteria and Actinobacteria were the dominant bacteria; at the genus level, was the dominant bacteria. Fish fed the diet containing 10% lipid had a higher abundance of Proteobacteria and than those fed the 14% lipid diet, and a higher abundance of than those fed the 6% lipid diet ( < 0.05). Analysis of the predicted functions showed that metabolism in the intestine of fish in the CP40EE10 group was more active than that in CP30EE14 group. Polynomial regression analysis found that a diet containing 40.87% protein and 9.88% lipid can be considered optimal for .
本研究旨在探讨日粮蛋白质和脂肪水平对[具体对象]生长性能及肠道菌群稳态的影响。进行了一项为期8周的3×3双因素试验，以研究日粮粗蛋白（CP：30%、35%、40%）和乙醚提取物（EE：6%、10%、14%）对[具体对象]生长速率和肠道微生物群的相互作用。总共2160尾鱼（5.19±0.01克）被随机分配到36个水族箱中，每个水族箱60尾鱼。每天给鱼投喂两次试验饲料。结果显示，当蛋白质水平从30%提高到40%时，增重率（WGR）、特定生长率（SGR）、蛋白质效率比和净蛋白质利用率显著提高（P<0.05）。随着日粮脂肪从6%增加到14%，WGR和SGR均先升高后降低，在10%脂肪水平时达到最大值（P<0.05）。在饲料转化率、脂肪效率比和净脂肪利用率方面发现蛋白质和脂肪之间存在显著相互作用（P<0.05）。在门水平上，变形菌门和放线菌门是优势菌；在属水平上，[具体属名]是优势菌。饲喂含10%脂肪日粮的鱼比饲喂含14%脂肪日粮的鱼具有更高丰度的变形菌门和[具体属名]，比饲喂含6%脂肪日粮的鱼具有更高丰度的[具体属名]（P<0.05）。预测功能分析表明，CP40EE10组鱼肠道中的代谢比CP30EE14组更活跃。多项式回归分析发现，含40.87%蛋白质和9.88%脂肪的日粮对[具体对象]可被认为是最佳的。
