Wang Ying, Zhang Jinhua, Zhou Qiubai, Wang Zirui, Gao Miao, Yang Xin, Liu Yu, Zhang Zhengzhou, Jiang Wenhao, Hu Chonghua, Zhang Wenping
Aquatic Animal Research Laboratory, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
Front Microbiol. 2020 Apr 30;11:699. doi: 10.3389/fmicb.2020.00699. eCollection 2020.
This study aimed to analyze the intestinal flora of male versus female swamp eels, which have the unique characteristic of sex reversal. Same-aged swamp eels from the same parents, kept under the same conditions in terms of environment, diet, etc., were used as the study materials. After breeding for 1 year, 18 healthy swamp eels (nine males and nine females), weighing 39.4 ± 2.3 g, were selected. The intestinal contents of three swamp eels of the same sex were combined and labeled as follows: FM1-FM3 ( = 3) for females and MM1-MM3 ( = 3) for males. High-throughput sequencing was used to research the v3-v4 region of 16S rDNA in the intestinal flora. The results suggested significant differences in the structure, composition, and diversity of the intestinal flora of male versus female swamp eels. The relative abundances of Actinobacteria and Spirochaetes in female swamp eels were significantly higher ( < 0.05) than in male swamp eels at the phylum level. The relative abundances of , , and in female swamp eels were considerably higher ( < 0.05) than in male swamp eels at the genus level. The Alpha diversity of females was higher than that of males, and the Shannon index of females was also significantly higher ( < 0.05, Tukey's HSD) than that in males. Investigations of Beta diversity, including NMDS ordination, UPGMA cluster analysis, and PLS-DA analysis, showed that female and male swamp eels could be clearly distinguished based on significant differences in intestinal flora between the FM group and the MM group. LEfSe analysis showed that the dominant bacteria were in male swamp eels and , , , and in female swamp eels. The results showed dramatic differences in the intestinal flora between the sexes, which indicates the need for a more in-depth study on sex reversal in the future.
本研究旨在分析具有性逆转这一独特特征的雌雄黄鳝的肠道菌群。选用来自同一亲本、在环境、饮食等相同条件下饲养1年的同龄黄鳝作为研究材料。饲养1年后，挑选出18条健康黄鳝（9条雄性和9条雌性），体重为39.4±2.3克。将同性别的3条黄鳝的肠道内容物合并，并作如下标记：雌性为FM1 - FM3（n = 3），雄性为MM1 - MM3（n = 3）。采用高通量测序技术研究肠道菌群中16S rDNA的v3 - v4区域。结果表明，雌雄黄鳝肠道菌群在结构、组成和多样性方面存在显著差异。在门水平上，雌性黄鳝中放线菌门和螺旋体门的相对丰度显著高于雄性黄鳝（P < 0.05）。在属水平上，雌性黄鳝中[此处原文缺失属名]、[此处原文缺失属名]和[此处原文缺失属名]的相对丰度显著高于雄性黄鳝（P < 0.05）。雌性的α多样性高于雄性，雌性的香农指数也显著高于雄性（P < 0.05，Tukey氏HSD检验）。包括非度量多维尺度排序（NMDS）、加权组平均法（UPGMA）聚类分析和偏最小二乘判别分析（PLS - DA）在内的β多样性研究表明，基于FM组和MM组肠道菌群的显著差异，可以清晰区分雌雄黄鳝。线性判别分析效应大小（LEfSe）分析表明，雄性黄鳝中的优势菌为[此处原文缺失菌名]，雌性黄鳝中的优势菌为[此处原文缺失菌名]、[此处原文缺失菌名]、[此处原文缺失菌名]和[此处原文缺失菌名]。结果显示，雌雄黄鳝的肠道菌群存在显著差异，这表明未来需要对性逆转进行更深入的研究。
