Nofima (Norwegian Institute of Food, Fisheries and Aquaculture Research), 1432Ås, Norway.
Epax Norway, 6006Ålesund, Norway.
Br J Nutr. 2023 Dec 14;130(11):1915-1931. doi: 10.1017/S0007114523001150. Epub 2023 May 12.
The purpose of this study was to investigate the effect of dietary -3 very-long-chain PUFA (-3 VLC-PUFA) on the maturation and development of skin tissue in juvenile Atlantic salmon r) , as well as their effects on skin keratocyte and human skin fibroblast cell migration Atlantic salmon were fed different dietary levels of -3 VLC-PUFA from an initial weight of 6 g to a final weight of 11 g. Changes in skin morphology were analysed at two time points during the experiment, and the effects on skin tissue fatty acid composition were determined. Additionally, experiments using human dermal fibroblasts and primary Atlantic salmon keratocytes were conducted to investigate the effect of VLC-PUFA on the migration capacity of the cells. The results demonstrated that increased dietary levels of -3 VLC-PUFA led to an increased epidermis thickness and more rapid scale maturation in Atlantic salmon skin , leading to a more mature skin morphology, and possibly more robust skin, at an earlier life stage. Additionally, human skin fibroblasts and salmon skin keratocytes supplemented with -3 VLC-PUFA showed more rapid migration, indicating potentially beneficial effects of VLC-PUFA in wound healing. In conclusion, VLC-PUFA may have beneficial effects on skin tissue development, function and integrity.
本研究旨在探讨饮食中 -3 超长链多不饱和脂肪酸（-3 VLC-PUFA）对大西洋鲑幼鱼皮肤组织成熟和发育的影响，以及其对皮肤角质细胞和人皮肤成纤维细胞迁移的影响。从初始体重 6 克到最终体重 11 克，大西洋鲑被喂食不同水平的 -3 VLC-PUFA。在实验过程中的两个时间点分析皮肤形态的变化，并确定皮肤组织脂肪酸组成的影响。此外，还进行了使用人真皮成纤维细胞和原代大西洋鲑角膜细胞的实验，以研究 VLC-PUFA 对细胞迁移能力的影响。结果表明，饮食中 -3 VLC-PUFA 水平的增加导致大西洋鲑皮肤表皮厚度增加，鳞片成熟更快，从而使皮肤形态更早达到更成熟的状态，皮肤可能更健壮。此外，补充了 -3 VLC-PUFA 的人皮肤成纤维细胞和鲑鱼皮肤角质细胞表现出更快的迁移，表明 VLC-PUFA 对伤口愈合可能有有益的影响。总之，VLC-PUFA 可能对皮肤组织的发育、功能和完整性具有有益的影响。
