Torrissen Martina, Ytteborg Elisabeth, Svensen Harald, Østbye Tone-Kari, Berge Gerd, Reppe Sjur, Krasnov Aleksei, Ruyter Bente
Nofima (Norwegian Institute of Food, Fisheries and Aquaculture Research), Ås1432, Norway.
Epax Norway, Ålesund6006, Norway.
Br J Nutr. 2025 Jan 28;133(2):145-160. doi: 10.1017/S0007114524003052. Epub 2024 Nov 29.
The present study investigated whether dietary -3 very-long-chain PUFA (-3 VLC-PUFA) could increase skin and bone mineralisation in Atlantic salmon () and examined their potential effects on human osteoblast proliferation and differentiation . Atlantic salmon were fed different dietary levels of -3 VLC-PUFA, and changes in tissue -3 VLC-PUFA composition, skeletal morphology, skin and bone mineral content, bone mineral density (BMD) and gene expression patterns were examined. Additionally, experiments using human foetal osteoblast cells were conducted to investigate the potential influence of -3 VLC-PUFA supplementation on cell proliferation, osteogenic differentiation and cytokine expression. The results demonstrated that increasing the dietary levels of -3 VLC-PUFA increased the mineral content of vertebrae and BMD in salmon, with subtle yet significant impacts on the expression of genes involved in bone-related processes. Furthermore, experiments showed a potential contextual influence of -3 VLC-PUFA supplementation on gene expression of osteogenic markers and cytokine expression. Our findings indicate for the first time that -3 VLC-PUFA may influence processes related to bone mineralisation.
本研究调查了膳食中的ω-3超长链多不饱和脂肪酸（ω-3 VLC-PUFA）是否能增加大西洋鲑（Salmo salar）的皮肤和骨骼矿化，并研究了它们对人成骨细胞增殖和分化的潜在影响。给大西洋鲑投喂不同水平的ω-3 VLC-PUFA，检测组织中ω-3 VLC-PUFA组成、骨骼形态、皮肤和骨骼矿物质含量、骨矿物质密度（BMD）以及基因表达模式的变化。此外，进行了使用人胎儿成骨细胞的实验，以研究补充ω-3 VLC-PUFA对细胞增殖、成骨分化和细胞因子表达的潜在影响。结果表明，增加膳食中ω-3 VLC-PUFA的水平可提高鲑鱼椎骨的矿物质含量和BMD，并对参与骨骼相关过程的基因表达产生细微但显著的影响。此外，实验表明补充ω-3 VLC-PUFA对成骨标志物的基因表达和细胞因子表达可能存在背景影响。我们的研究结果首次表明，ω-3 VLC-PUFA可能影响与骨骼矿化相关的过程。
