Weisweiler P, Janetschek P, Schwandt P
Am J Clin Nutr. 1986 Jun;43(6):903-9. doi: 10.1093/ajcn/43.6.903.
We investigated effects of fat saturation and fat restriction on very low-density apolipoproteins (VLDL) including the isoforms. Normolipidemic women (22) were given a reference diet, a polyunsaturated diet, and a low-fat, polyunsaturated diet for 6 wk each. The polyunsaturated diet decreased cholesterol and apolipoprotein B levels in VLDL (-33.1% and -23.8%) and in LDL (-13.5% and -8.8%) without affecting HDL. The low-fat, polyunsaturated diet resulted in a reincrease of VLDL triglycerides, but not of VLDL cholesterol. Concentration of VLDL apolipoprotein B fell further (-41.6%). All VLDL apolipoprotein B was in the B-100 region. Though the apolipoprotein E phenotype (E-3/E-3) remained constant, a shift to more (nonsialated) apolipoprotein E isoforms could be confirmed, resulting in an increased apolipoprotein E-3 to apolipoprotein E-2 area ratio (+30.6%). This study indicates that restriction of dietary-fat intake alters the composition of apolipoprotein B-100 containing VLDL that may be favorable for atherogenesis.
我们研究了脂肪饱和度和脂肪限制对包括亚型在内的极低密度载脂蛋白（VLDL）的影响。给22名血脂正常的女性分别提供标准饮食、多不饱和饮食和低脂多不饱和饮食，每种饮食持续6周。多不饱和饮食可降低VLDL中的胆固醇和载脂蛋白B水平（分别降低33.1%和23.8%）以及LDL中的胆固醇和载脂蛋白B水平（分别降低13.5%和8.8%），而对HDL没有影响。低脂多不饱和饮食导致VLDL甘油三酯再次升高，但VLDL胆固醇没有升高。VLDL载脂蛋白B的浓度进一步下降（降低41.6%）。所有VLDL载脂蛋白B都在B-100区域。虽然载脂蛋白E表型（E-3/E-3）保持不变，但可以确认向更多（非唾液酸化）载脂蛋白E亚型转变，导致载脂蛋白E-3与载脂蛋白E-2面积比增加（增加30.6%）。这项研究表明，饮食脂肪摄入的限制会改变含载脂蛋白B-100的VLDL的组成，这可能对动脉粥样硬化的发生发展有利。
