Weisweiler P, Janetschek P, Schwandt P
Metabolism. 1985 Jan;34(1):83-7. doi: 10.1016/0026-0495(85)90065-4.
This study was designed to determine the effects of polyunsaturated fats and of reducing intake of total fat on serum lipids, lipoproteins, and apolipoproteins. Twenty-two normolipidemic women living in a nunnery were given a reference diet (fat/carbohydrate 42/46% of energy, P/S ratio 0.16), a polyunsaturated diet (42/46%, P/S 1,0), and a low-fat, polyunsaturated diet (32/56%, P/S 1.0) for 6 weeks each. Serum and lipoprotein lipids were determined by standard procedures, apolipoproteins either by laser immunonephelometry or by rocket immunoelectrophoresis. Consumption of the polyunsaturated diet decreased cholesterol and apolipoprotein B levels in VLDL (-33.1% and -23.8%) and in LDL (-13.5% and -8.8%) without affecting HDL. Consumption of the low-fat, polyunsaturated diet resulted in a reincrease of VLDL triglycerides, but not of VLDL cholesterol. Concentration of VLDL apolipoprotein B further fell (-41.6%) and that of apolipoprotein E decreased (-25.9%), resulting in an increased VLDL lipid/apolipoprotein mass ratio. This study indicates that responses to therapeutic polyunsaturated diet are lowered levels of VLDL and LDL, but unchanged levels of HDL. Additional restriction of dietary fat intake alters the VLDL composition with a decrement in apolipoprotein E enriched VLDL particles.
本研究旨在确定多不饱和脂肪以及减少总脂肪摄入量对血清脂质、脂蛋白和载脂蛋白的影响。22名居住在尼姑庵的血脂正常女性分别接受了6周的参考饮食（脂肪/碳水化合物占能量的42/46%，P/S比值0.16）、多不饱和饮食（42/46%，P/S 1.0）和低脂多不饱和饮食（32/56%，P/S 1.0）。血清和脂蛋白脂质通过标准程序测定，载脂蛋白通过激光免疫比浊法或火箭免疫电泳法测定。食用多不饱和饮食可降低极低密度脂蛋白（VLDL）（-33.1%和-23.8%）和低密度脂蛋白（LDL）（-13.5%和-8.8%）中的胆固醇和载脂蛋白B水平，而不影响高密度脂蛋白（HDL）。食用低脂多不饱和饮食导致VLDL甘油三酯再次升高，但VLDL胆固醇未升高。VLDL载脂蛋白B的浓度进一步下降（-41.6%），载脂蛋白E的浓度下降（-25.9%），导致VLDL脂质/载脂蛋白质量比增加。本研究表明，对治疗性多不饱和饮食的反应是VLDL和LDL水平降低，但HDL水平不变。进一步限制饮食脂肪摄入量会改变VLDL组成，富含载脂蛋白E的VLDL颗粒减少。
