Sanya Nanfan Research Institute of Hainan University, Sanya, China.
Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, China.
Physiol Plant. 2023 May-Jun;175(3):e13932. doi: 10.1111/ppl.13932.
Sodium nitroprusside (SNP), as a nitric oxide donor, is widely used in postharvest fruit physiology and metabolism. Our previous study has indicated that SNP plays a crucial role in postharvest browning control of rambutan, but the molecular mechanism underlying this process is still unclear. In this research, we investigated the gene expression and function of postharvest rambutan in response to SNP during browning. We found 7336 differentially expressed genes (DEGs), among which 2206 were upregulated and 5130 were downregulated. Gene Ontology (GO) enrichment as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed, and the real-time quantitative PCR (qPCR) data were consistent with transcriptome data. The DEGs relevant to rambutan pericarp browning were mainly involved in anthocyanin biosynthesis, phenolic oxidation, reactive oxygen species (ROS) production, and energy supply. It was shown that SNP regulated the synthesis and degradation of anthocyanins, accumulation of phenols, level of ROS and energy metabolism to suppress the postharvest browning of rambutan. Also, one WRKY transcription factor involved in ROS metabolism was observed to be differentially regulated. These findings add to our insights into the molecular mechanisms of the SNP-induced browning delays of rambutan, which has implications for subsequent studies on molecular mechanisms of fruit browning.
硝普钠（SNP）作为一种一氧化氮供体，广泛应用于采后果实生理学和代谢研究。我们之前的研究表明 SNP 在荔枝采后褐变控制中起着关键作用，但这一过程的分子机制尚不清楚。在这项研究中，我们研究了 SNP 处理对荔枝采后褐变相关基因表达和功能的影响。我们发现了 7336 个差异表达基因（DEGs），其中 2206 个上调，5130 个下调。进行了基因本体（GO）富集和京都基因与基因组百科全书（KEGG）分析，实时定量 PCR（qPCR）数据与转录组数据一致。与荔枝果皮褐变相关的 DEGs 主要涉及类黄酮生物合成、酚类氧化、活性氧（ROS）产生和能量供应。结果表明，SNP 通过调节花色苷的合成和降解、酚类物质的积累、ROS 水平和能量代谢来抑制荔枝的采后褐变。此外，观察到一个参与 ROS 代谢的 WRKY 转录因子也被差异调控。这些发现增加了我们对 SNP 诱导荔枝褐变延迟的分子机制的认识，这对后续研究果实褐变的分子机制具有重要意义。
