Elsebaie Essam Mohamed, El-Wakeil Nora Hamdy Mouhamed, Khalil Azhar Mostafa Mohamed, Bahnasy Rasha M, Asker Galila Ali, El-Hassnin Marwa Fawzy, Ibraheim Suzan S, El-Farsy Marwa Fawzi Ahmed, Faramawy Asmaa Antar, Essa Rowida Younis, Badr Mohamed Reda
Food Technology Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Nutrition &Food Science Department, Faculty of Home Economics, Al-Azhar University, Tanta 31512, Egypt.
Foods. 2023 Apr 23;12(9):1746. doi: 10.3390/foods12091746.
The consumption of foods polluted with different foodborne pathogens such as fungus, viruses, and bacteria is considered a serious cause of foodborne disease in both humans and animals. Multidrug-resistant foodborne pathogens (MRFP) cause morbidity, death, and substantial economic loss, as well as prolonged hospitalization. This study reports on the use of aqueous leaf extract (ARLE) in the synthesis of silver nanoparticles (ARLE-AgNPs) with versatile biological activities. The synthesized ARLE-AgNPs had spherical shapes with smooth surfaces and an average hydrodynamic size of 27 nm. ARLE-AgNPs inhibited the growth of ATCC25721, ATCC27843, ATCC49716, ATCC700813, and ATCC4342. The ARLE-AgNPs were more active against ATCC25721 than other harmful bacterial strains (26 ± 3 mm). The zone of inhibition for antibacterial activity ranged between 18 ± 3 mm and 26 ± 3 mm in diameter. The nanoparticles' MIC values varied from 5.19 µg/mL to 61 µg/mL, while their MBC values ranged from 46 µg/mL to 119 µg/mL. The nanoparticles that were created had antioxidant potential. The cytotoxic activity was tested using normal fibroblast cell lines (L-929), and the enhanced IC50 value (764.3 ± 3.9 g/mL) demonstrated good biological compatibility. These nanoparticles could be evolved into new antibacterial compounds for MRFP prevention.
食用被真菌、病毒和细菌等不同食源性病原体污染的食物被认为是人类和动物食源性疾病的一个严重原因。多重耐药食源性病原体（MRFP）会导致发病、死亡、巨大的经济损失以及住院时间延长。本研究报告了使用水提叶提取物（ARLE）合成具有多种生物活性的银纳米颗粒（ARLE-AgNPs）。合成的ARLE-AgNPs呈球形，表面光滑，平均流体动力学尺寸为27nm。ARLE-AgNPs抑制了ATCC25721、ATCC27843、ATCC49716、ATCC700813和ATCC4342的生长。ARLE-AgNPs对ATCC25721的活性比对其他有害细菌菌株更强（26±3mm）。抗菌活性的抑菌圈直径在18±3mm至26±3mm之间。纳米颗粒的MIC值在5.19μg/mL至61μg/mL之间，而其MBC值在46μg/mL至119μg/mL之间。所制备的纳米颗粒具有抗氧化潜力。使用正常成纤维细胞系（L-929）测试细胞毒性活性，增强的IC50值（764.3±3.9μg/mL）表明具有良好的生物相容性。这些纳米颗粒可以发展成为预防MRFP的新型抗菌化合物。
