Participation of FaTRAB1 Transcription Factor in the Regulation of Involved in ABA-Dependent Ripening of Strawberry Fruit.

Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of . The transient silence of in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco rattle virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related , , , , and , softening-related and , and aroma-related and In addition, the interaction between and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the promoter could interact with FaABI5-5, FaTRAB1, and FaABI5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the promoter, resulting in the decreased expression of . In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting expression by the active binding of transcript FaTRAB1 with the promoter.