Li Dongdong, Mou Wangshu, Xia Rui, Li Li, Zawora Christopher, Ying Tiejin, Mao Linchun, Liu Zhongchi, Luo Zisheng
1College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agri-Food Processing, Zhejiang University, 310058 Hangzhou, P.R. China.
2Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742 USA.
Hortic Res. 2019 Feb 1;6:26. doi: 10.1038/s41438-018-0100-8. eCollection 2019.
The perception and signal transduction of the plant hormone abscisic acid (ABA) are crucial for strawberry fruit ripening, but the underlying mechanism of how ABA regulates ripening-related genes has not been well understood. By employing high-throughput sequencing technology, we comprehensively analyzed transcriptomic and miRNA expression profiles simultaneously in ABA- and nordihydroguaiaretic acid (NDGA, an ABA biosynthesis blocker)-treated strawberry fruits with temporal resolution. The results revealed that ABA regulated many genes in different pathways, including hormone signal transduction and the biosynthesis of secondary metabolites. Transcription factor genes belonging to WRKY and heat shock factor (HSF) families might play key roles in regulating the expression of ABA inducible genes, whereas the KNOTTED1-like homeobox protein and Squamosa Promoter-Binding-like protein 18 might be responsible for ABA-downregulated genes. Additionally, 20 known and six novel differentially expressed miRNAs might be important regulators that assist ABA in regulating target genes that are involved in versatile physiological processes, such as hormone balance regulation, pigments formation and cell wall degradation. Furthermore, degradome analysis showed that one novel miRNA, Fa_novel6, could degrade its target gene , which likely contributed to fruit size determination during strawberry ripening. These results expanded our understanding of how ABA drives the strawberry fruit ripening process as well as the role of miRNAs in this process.
植物激素脱落酸（ABA）的感知和信号转导对草莓果实成熟至关重要，但ABA如何调控成熟相关基因的潜在机制尚未完全清楚。通过采用高通量测序技术，我们在经ABA和去甲二氢愈创木酸（NDGA，一种ABA生物合成阻滞剂）处理的草莓果实中，以时间分辨率同时全面分析了转录组和miRNA表达谱。结果表明，ABA调控了不同途径中的许多基因，包括激素信号转导和次生代谢产物的生物合成。属于WRKY和热激因子（HSF）家族的转录因子基因可能在调控ABA诱导基因的表达中起关键作用，而类KNOTTED1同源异型盒蛋白和类Squamosa启动子结合蛋白18可能负责ABA下调的基因。此外，20个已知的和6个新的差异表达miRNA可能是重要的调节因子，协助ABA调控参与多种生理过程的靶基因，如激素平衡调节、色素形成和细胞壁降解。此外，降解组分析表明，一种新的miRNA，Fa_novel6，可以降解其靶基因，这可能有助于草莓成熟过程中果实大小的决定。这些结果扩展了我们对ABA如何驱动草莓果实成熟过程以及miRNA在此过程中的作用的理解。
