Ponce María Del Valle, Cina Mariel, López Carlos, Cerutti Soledad
Instituto de Química de San Luis (INQUISAL-CONICET-UNSL), Laboratorio de Espectrometría de Masas, Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Bloque III, Ejército de los Andes 950, San Luis 5700, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, Buenos Aires 1033, Argentina.
Foods. 2023 Apr 28;12(9):1828. doi: 10.3390/foods12091828.
A novel solid-phase extraction methodology followed by UHPLC-MS/MS has been developed for Ochratoxin A (OTA) analysis in herbal infusions. For this purpose, a commercial polyurethane foam (PUF) was used as sorbent, and the experimental conditions were fully optimized. The strategy was satisfactory for reducing the matrix effect and allowed for OTA quantification in black tea and herbal infusions, with suitable recoveries and quantitation limits in agreement with those required by the maximum levels allowed by current regulations. The achieved results demonstrated the unprecedented use of polyurethane foam as an effective alternative for OTA retention and quantification in herbal infusions with the advantages of simple preparation, time saving, sustainability, and low cost for routine analysis.
一种新型的固相萃取方法,随后采用超高效液相色谱-串联质谱法,已被开发用于分析草药浸液中的赭曲霉毒素A(OTA)。为此,使用了一种商用聚氨酯泡沫(PUF)作为吸附剂,并对实验条件进行了全面优化。该策略在降低基质效应方面令人满意,能够对红茶和草药浸液中的OTA进行定量,回收率和定量限合适,符合现行法规允许的最大限量要求。所取得的结果表明,聚氨酯泡沫前所未有的被用作一种有效的替代品,用于在草药浸液中保留和定量OTA,具有制备简单、节省时间、可持续性以及常规分析成本低等优点。