Cina Mariel, Ponce María Del Valle, Martinez Luis Dante, Cerutti Soledad
Instituto de Química de San Luis (CCT-San Luis), Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Laboratorio de Espectrometría de Masas, Bloque III, Ejército de los Andes 950, San Luis, CP5700, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina.
Heliyon. 2021 Apr 8;7(4):e06663. doi: 10.1016/j.heliyon.2021.e06663. eCollection 2021 Apr.
The mycotoxin Ochratoxin A (OTA) is responsible for producing many effects on human and animal health. In this work, the evaluation of the presence of OTA in tea beverage samples consisted of extraction and preconcentration through the solidification of a floating organic drop (DLLME-SFO) combined with an additional octadecyl silane clean-up step. The obtained extract was analyzed by UHPLC-MS/MS. Interferences from the matrix were effectively reduced and, consequently, recovery increased from 43.18% ± 4.1%-96.02% ± 2.54%. The validation assays were carried out by external calibration and spiked samples, with satisfactory recoveries. An adequate dynamic calibration range was obtained over a concentration interval between 0.5 and 70 μg mL OTA. Capabilities of detection and quantification were 0.5 and 1.4 μg mL. The obtained Green Certificate was compared with other techniques to establish the greenness profile of the procedure. Quantification of ochratoxin A levels in tea samples was performed.
霉菌毒素赭曲霉毒素A（OTA）对人类和动物健康会产生多种影响。在本研究中，对茶饮料样品中OTA的检测包括通过浮动有机滴固化萃取和预富集（分散液液微萃取-固化漂浮有机滴，DLLME-SFO），并结合额外的十八烷基硅烷净化步骤。所得提取物采用超高效液相色谱-串联质谱法（UHPLC-MS/MS）进行分析。基质干扰得到有效降低，回收率从43.18%±4.1%提高到96.02%±2.54%。通过外标法和加标样品进行验证试验，回收率令人满意。在0.5至70μg/mL OTA的浓度区间内获得了合适的动态校准范围。检测限和定量限分别为0.5和1.4μg/mL。将所得绿色认证与其他技术进行比较，以确定该方法的绿色度概况。对茶样品中赭曲霉毒素A的含量进行了定量分析。
