Stem Cell Research Center, Department of Hematology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, India.
Department of Hematology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, India.
Cells. 2023 May 2;12(9):1303. doi: 10.3390/cells12091303.
Erythropoiesis is a highly regulated process and undergoes several genotypic and phenotypic changes during differentiation. The phenotypic changes can be evaluated using a combination of cell surface markers expressed at different cellular stages of erythropoiesis using FACS. However, limited studies are available on the in-depth phenotypic characterization of progenitors from human adult hematopoietic stem and progenitor cells (HSPCs) to red blood cells. Therefore, using a set of designed marker panels, in the current study we have kinetically characterized the hematopoietic, erythroid progenitors, and terminally differentiated erythroblasts ex vivo. Furthermore, the progenitor stages were explored for expression of CD117, CD31, CD41a, CD133, and CD45, along with known key markers CD36, CD71, CD105, and GPA. Additionally, we used these marker panels to study the stage-specific phenotypic changes regulated by the epigenetic regulator; () during erythropoiesis and to study ineffective erythropoiesis in myelodysplastic syndrome (MDS) and pure red cell aplasia (PRCA) patients. Our immunophenotyping strategy can be used to sort and study erythroid-primed hematopoietic and erythroid precursors at specified time points and to study diseases resulting from erythroid dyspoiesis. Overall, the current study explores the in-depth kinetics of phenotypic changes occurring during human erythropoiesis and applies this strategy to study normal and defective erythropoiesis.
红细胞生成是一个高度受调控的过程，在分化过程中经历了几种基因型和表型的改变。表型的改变可以通过使用流式细胞术（FACS）在红细胞生成的不同细胞阶段表达的细胞表面标志物的组合来评估。然而，关于从人类成人造血干细胞和祖细胞（HSPCs）到红细胞的祖细胞的深入表型特征的研究有限。因此，在当前研究中，我们使用一组设计的标记面板，从动力学上对造血细胞、红细胞祖细胞和终末分化的红细胞进行了体外特征分析。此外，还探索了祖细胞阶段 CD117、CD31、CD41a、CD133 和 CD45 的表达情况，以及已知的关键标记物 CD36、CD71、CD105 和 GPA。此外，我们还使用这些标记面板研究了红细胞生成过程中由表观遗传调节剂调控的阶段特异性表型变化，并研究了骨髓增生异常综合征（MDS）和纯红细胞再生障碍性贫血（PRCA）患者无效红细胞生成的情况。我们的免疫表型策略可用于在特定时间点对造血和红细胞前体细胞进行分类和研究，并用于研究由于红细胞生成异常引起的疾病。总的来说，本研究探讨了人类红细胞生成过程中发生的表型变化的深入动力学，并将该策略应用于研究正常和异常的红细胞生成。
