Hedges Sophie, Mompelat Sophie, Hurtaud-Pessel Dominique, Blake Damer P, Fournié Guillaume, Pelligand Ludovic
The Royal Veterinary College, Hatfield, UK.
London School of Hygiene & Tropical Medicine, London, UK.
J Vet Pharmacol Ther. 2025 Sep;48(5):405-416. doi: 10.1111/jvp.70000. Epub 2025 Jun 3.
Persistence of antimicrobial drugs (AMDs) administered to poultry is longer in feathers than in edible tissues. Hence, poultry feathers are a suitable matrix to investigate historical exposure contributing to antimicrobial resistance, since current detection methods are either non-specific or highly technical and costly. Here we present an analysis of the performance of lateral flow test (LFT) panels in the detection of five AMD classes, namely sulfonamides, tetracyclines, beta-lactams, quinolones, and aminoglycosides, on chicken feather samples. The limit of detection (LOD) of eight AMD substances was determined between 4.7 μg/kg for enrofloxacin and 700 μg/kg for streptomycin. The performance of feather LFT was evaluated for four AMD classes against the reference method (LC-MS/MS). From 79 samples collected from the field, LFT test specificity ranged from 0.63 (quinolones) to 0.95 (tetracyclines). Test sensitivity ranged from 0.15 (beta-lactams) to 0.78 (quinolones and tetracyclines). LFT testing had the greatest discriminatory power for tetracyclines (specificity 0.95 and sensitivity 0.78). LFT had similar test characteristics for sulfonamides and quinolones and performed poorly for beta-lactams. Poor recovery rates (< 15%) were observed in neomycin, kanamycin, and ampicillin. These methods are suitable for preliminarily screening tetracyclines, sulfonamides, and quinolones, with recommendations for further extraction protocols.
家禽体内施用的抗菌药物（AMDs）在羽毛中的残留时间比在可食用组织中更长。因此，家禽羽毛是一种合适的基质，可用于调查导致抗菌药物耐药性的历史暴露情况，因为目前的检测方法要么是非特异性的，要么技术要求高且成本高昂。在此，我们分析了侧向流动检测（LFT）板对鸡毛样本中五类AMDs（即磺胺类、四环素类、β-内酰胺类、喹诺酮类和氨基糖苷类）的检测性能。测定了八种AMD物质的检测限（LOD），恩诺沙星为4.7μg/kg，链霉素为700μg/kg。针对四类AMD，将羽毛LFT的性能与参考方法（液相色谱-质谱/质谱法）进行了评估。从现场采集的79个样本中，LFT检测的特异性范围为0.63（喹诺酮类）至0.95（四环素类）。检测灵敏度范围为0.15（β-内酰胺类）至0.78（喹诺酮类和四环素类）。LFT检测对四环素类的鉴别能力最强（特异性0.95，灵敏度0.78）。LFT对磺胺类和喹诺酮类具有相似的检测特性，而对β-内酰胺类的检测效果较差。在新霉素、卡那霉素和氨苄青霉素中观察到回收率较低（<15%）。这些方法适用于四环素类、磺胺类和喹诺酮类的初步筛查，并对进一步的提取方案提出了建议。
