一种制备[32P]磷脂酸的方法。

A procedure for the preparation of [32P]phosphatidic acid.

作者信息

Keller R K, Adair W L, Cafmeyer N

出版信息

Anal Biochem. 1986 May 15;155(1):119-22. doi: 10.1016/0003-2697(86)90235-6.

Abstract

The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in 2 h. The product was shown to be radiochemically pure by both TLC and HPLC. L- and DL-[32P]dipalmitoyl phosphatidic acid prepared using this procedure were shown to be hydrolyzed by rat liver microsomes at approximately the same rates.

摘要

F. 克莱默、W. 里特斯多夫和W. 博姆 [（1961年）《化学学报》654, 180] 采用磷酸双（三乙铵）和三氯乙腈的磷酸化程序，在[32P]磷脂酸的合成中被证明是有效的。由二酰甘油和0.5毫居里的H(3)32PO4，可在2小时内制备出25 - 50微居里的标记物质（比活度 = 1毫居里/微摩尔）。通过薄层层析（TLC）和高效液相色谱（HPLC）证明该产物在放射化学上是纯的。使用此程序制备的L-和DL-[32P]二棕榈酰磷脂酸被证明能被大鼠肝脏微粒体以大致相同的速率水解。