A biosynthetic method for the preparation of high specific activity 32P-labeled phospholipids.
作者信息
Marggraf W D
出版信息
J Lipid Res. 1976 Nov;17(6):622-8.
PMID:993672
Abstract
32P-Labeled phospholipids with specific activities up to 400 mCi/mmole as well as [32P]CDP-choline were prepared by cultivation of mouse fibroblasts or mouse Ehrlich ascites cells in the presence of [32P]orthophosphate. The method was also used to prepare [methyl-3H]choline-labeled glycerophospholipids from [3H]choline. The yields and the specific activities of the phospholipids were significantly lower when preparations of ox white blood cells were used.
摘要
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