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Measurements of serum glucose using the luciferin/luciferase system and a liquid scintillation spectrometer.

作者信息

Idahl L A, Sandström P E, Sehlin J

出版信息

Anal Biochem. 1986 May 15;155(1):177-81. doi: 10.1016/0003-2697(86)90243-5.

DOI:10.1016/0003-2697(86)90243-5
PMID:3717554
Abstract

A single-step assay for serum glucose measurements is described. The assay is based on the phosphorylation of D-glucose by glucokinase and the measurement of ATP consumption by firefly luciferase. The luminescence is recorded in an ordinary liquid scintillation spectrometer. The use of stable reagents and a stable final signal (light emission) makes it possible to analyze a large number of samples in each assay run. The assay is of particular value when repeated serum glucose determinations are performed on samples from small laboratory animals.

摘要

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Measurements of serum glucose using the luciferin/luciferase system and a liquid scintillation spectrometer.
Anal Biochem. 1986 May 15;155(1):177-81. doi: 10.1016/0003-2697(86)90243-5.
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ATP formation onset lag and post-illumination phosphorylation initiated with single-turnover flashes. I. An assay using luciferin-luciferase luminescence.ATP形成起始延迟和光照后磷酸化由单周转闪光引发。I. 使用荧光素-荧光素酶发光的测定法。
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