Tao Qibo, Chen Dali, Bai Mengjie, Zhang Yaqi, Zhang Ruizhen, Chen Xiaofei, Sun Xiaotong, Niu Tianxiu, Nie Yuting, Zhong Shangzhi, Sun Juan
Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China.
State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
Plants (Basel). 2023 May 4;12(9):1876. doi: 10.3390/plants12091876.
Seed vigor is an important aspect of seed quality. High-vigor seeds show rapid and uniform germination and emerge well, especially under adverse environmental conditions. Here, we determined hydrotime model parameters by incubating seeds at different water potentials (0.0, -0.2, -0.4, -0.6, and -0.8 MPa) in the laboratory, for 12 seed lots of Chinese milk vetch () (CMV), a globally important legume used as forage, green manure, and a rotation crop. Pot experiments were conducted to investigate the seedling emergence performance of 12 CMV seed lots under control, water stress, salinity stress, deep sowing, and cold stress conditions. Meanwhile, the field emergence performance was evaluated on two sowing dates in June and October 2022. Correlation and regression analyses were implemented to explore the relationships between hydrotime model parameters and seedling emergence performance under various environmental conditions. The seed germination percentage did not differ significantly between seed lots when seeds were incubated at 0.0 MPa, whereas it did differ significantly between seed lots at water potentials of -0.2, -0.4, and -0.6 MPa. The emergence percentage, seedling dry weight, and simplified vigor index also differed significantly between the 12 seed lots under various environmental conditions. showed a significant correlation with germination and emergence performance under various environmental conditions; however, little correlation was observed between or and germination and emergence. These results indicate that can be used to estimate seed vigor and predict seedling emergence performance under diverse environmental conditions for CMV and similar forage legumes. This study will enable seed researchers, plant breeders, and government program directors to target higher seed vigor more effectively for forage legumes.
种子活力是种子质量的一个重要方面。高活力种子发芽迅速且整齐，出苗良好，尤其是在不利的环境条件下。在此，我们通过在实验室中于不同水势（0.0、-0.2、-0.4、-0.6和-0.8兆帕）下培养种子，来确定12个紫云英种子批次的水时模型参数。紫云英是一种全球重要的豆科植物，用作饲料、绿肥和轮作作物。进行盆栽试验以研究12个紫云英种子批次在对照、水分胁迫、盐分胁迫、深播和低温胁迫条件下的出苗表现。同时，于2022年6月和10月的两个播种日期对田间出苗表现进行了评估。实施相关分析和回归分析以探究水时模型参数与各种环境条件下的出苗表现之间的关系。当种子在0.0兆帕下培养时，种子批次之间的发芽率没有显著差异，而在-0.2、-0.4和-0.6兆帕的水势下，种子批次之间的发芽率有显著差异。在各种环境条件下，12个种子批次之间的出苗率、幼苗干重和简化活力指数也有显著差异。在各种环境条件下，[此处原文缺失相关指标]与发芽和出苗表现显著相关；然而，[此处原文缺失相关指标]与发芽和出苗之间几乎没有相关性。这些结果表明，[此处原文缺失相关指标]可用于估计紫云英和类似饲料豆科植物在不同环境条件下的种子活力并预测出苗表现。本研究将使种子研究人员、植物育种者和政府项目负责人能够更有效地针对饲料豆科植物提高种子活力。
