State Key Laboratory of Grassland Agro-Ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; College of Pastoral Agricult, Lanzhou, China.
PeerJ. 2022 May 10;10:e13206. doi: 10.7717/peerj.13206. eCollection 2022.
We determined the hydrotime model parameters of 10 alfalfa seed lots by incubating seeds at various water potentials in the laboratory. Meanwhile, seedling emergence under drought and salinity conditions in a greenhouse pot experiment, and seedling establishment in the field were determined. Correlation analysis was utilized to detect the relationship between hydrotime model parameters and seedling emergence under water stress conditions. The germination percentage did not differ significantly among seed lots when seeds were incubated at -0.1 MPa, while it differed significantly among seed lots at water potentials of -0.3 and -0.6 MPa. Compared to control conditions, drought and salinity decreased seedling emergence to different extents, depending on seed lots. Seedling emergence in the field differed significantly among seed lots and ranged from 30% to 80%. showed a significant correlation with seedling emergence under various conditions and with seedling establishment in the field, while no correlation was observed between , and seedling emergence and establishment. These results suggest that can be used to rank the vigor of alfalfa seed lots and thus predict seedling emergence and establishment under water stress conditions.
我们通过在实验室中在不同水势下孵育种子来确定 10 个紫花苜蓿种子批的水时模型参数。同时,在温室盆栽试验中确定干旱和盐胁迫条件下的幼苗出苗率,以及在田间的幼苗建立。相关性分析用于检测水时模型参数与水分胁迫条件下幼苗出苗率之间的关系。当种子在-0.1 MPa 下孵育时,种子批之间的发芽率没有显著差异,但在-0.3 和-0.6 MPa 的水势下,种子批之间的发芽率有显著差异。与对照条件相比,干旱和盐胁迫不同程度地降低了幼苗的出苗率,具体取决于种子批。田间的幼苗出苗率在种子批之间有显著差异,范围从 30%到 80%。水时模型参数与各种条件下的幼苗出苗率以及田间幼苗建立呈显著相关,而与种子出苗率和建立无关。这些结果表明,可以用来对紫花苜蓿种子批的活力进行排序,从而预测水分胁迫条件下的幼苗出苗率和建立。