Álvarez Fernanda, Moya Mario, Rivera-Mora Claudia, Zúñiga Paz E, Jara-Cornejo Karla, Muñoz Paula, Ayala-Raso Aníbal, Munné-Bosch Sergi, Figueroa Carlos R, Figueroa Nicolás E, Valdenegro Mónika, Alvaro Juan E, Schwab Wilfried, Defilippi Bruno G, Fuentes Lida
Centro Regional de Estudios en Alimentos Saludables (CREAS), CONICYT-Regional GORE Valparaíso Proyecto R17A10001, Avenida Universidad 330, Placilla, Curauma, Valparaíso 2362696, Chile.
Laboratory of Plant Molecular Physiology, Institute of Biological Sciences, Universidad de Talca, Talca 3465548, Chile.
Plants (Basel). 2023 May 5;12(9):1882. doi: 10.3390/plants12091882.
The raspberry ( L.) fruit is characterized by its richness in functional molecules and high nutritional value, but the high rate of fruit softening limits its quality during postharvest. Raspberry drupelets have a particular ripening regulation, depending partially on the effect of ethylene produced from the receptacle. However, the possible role of abscisic acid (ABA) in the modulation of quality parameters during the ripening of raspberry is unclear. This study characterized the fruit quality-associated parameters and hormonal contents during fruit development in two seasons. The quality parameters showed typical changes during ripening: a drastic loss of firmness, increase in soluble solids content, loss of acidity, and turning to a red color from the large green stage to fully ripe fruit in both seasons. A significant increase in the ABA content was observed during the ripening of drupelets and receptacles, with the higher content in the receptacle of ripe and overripe stages compared to the large green stage. Moreover, identification of ABA biosynthesis-(9-cis-epoxycarotenoid dioxygenase/NCED) and ABA receptor-related genes (PYRs-like receptors) showed three genes encoding RiNCEDs and nine genes for RiPYLs. The expression level of these genes increased from the large green stage to the full-ripe stage, specifically characterized by a higher expression of in the receptacle tissue. This study reports a consistent concomitant increase in the ABA content and the expression of and during the ripening of the raspberry fruit, thus supporting the role for ABA signaling in drupelets.
树莓（L.）果实的特点是富含功能性分子且营养价值高，但果实软化率高限制了其采后的品质。树莓小核果具有特殊的成熟调控机制，部分取决于花托产生的乙烯的作用。然而，脱落酸（ABA）在树莓成熟过程中对品质参数调节的可能作用尚不清楚。本研究对两个季节果实发育过程中的果实品质相关参数和激素含量进行了表征。品质参数在成熟过程中呈现典型变化：两个季节中，果实硬度急剧下降、可溶性固形物含量增加、酸度降低，并且从大绿果期到完全成熟果实颜色从绿色变为红色。在小核果和花托成熟过程中观察到ABA含量显著增加，与大绿果期相比，成熟和过熟阶段花托中的含量更高。此外，对ABA生物合成相关基因（9-顺式环氧类胡萝卜素双加氧酶/NCED）和ABA受体相关基因（PYR类受体）的鉴定显示，有三个基因编码RiNCED，九个基因编码RiPYL。这些基因的表达水平从大绿果期到完全成熟阶段增加，其特点是在花托组织中 表达更高。本研究报道了树莓果实成熟过程中ABA含量以及 和 的表达一致地同时增加，从而支持了ABA信号在小核果中的作用。
