Khan Waqar, El-Shehawi Ahmed M, Ali Fayaz, Ali Murad, Alqurashi Mohammed, Althaqafi Mohammed M, Alharthi Siraj B
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Plants (Basel). 2023 May 7;12(9):1904. doi: 10.3390/plants12091904.
Laccase-like multi-copper oxidases (LMCOs) are a group of enzymes involved in the oxidation of numerous substrates. Recently, these enzymes have become extremely popular due to their practical applications in various fields of biology. LMCOs generally oxidize various substrates by linking four-electron reduction of the final acceptor, molecular oxygen (O), to water. Multi-copper oxidases related to laccase are extensively distributed as multi-gene families in the genome sequences of higher plants. The current study thoroughly investigated the LMCO gene family (Br-Lac) and its expression pattern under various abiotic stresses in L. A total of 18 Br-Lac gene family members located on five different chromosomes were identified. Phylogenetic analysis classified the documented Br-Lac genes into seven groups: Group-I (four genes), Group-II (nine genes), Group-III (eight genes), Group-IV (four genes), Group-V (six genes), and Group-VI and Group-VII (one gene each). The key features of gene structure and responsive motifs shared the utmost resemblance within the same groups. Additionally, a divergence study also assessed the evolutionary features of Br-Lac genes. The anticipated period of divergence ranged from 12.365 to 39.250 MYA (million years ago). We also identified the pivotal role of the 18 documented members of the LMCO (Br-lac) gene family using quantitative real-time qRT-PCR. Br-Lac-6, Br-Lac-7, Br-Lac-8, Br-Lac-16, Br-Lac-17, and Br-Lac-22 responded positively to abiotic stresses (i.e., drought, heat, and salinity). These findings set the stage for the functional diversity of the LMCO genes in .
漆酶样多铜氧化酶（LMCOs）是一类参与多种底物氧化的酶。最近，由于这些酶在生物学各个领域的实际应用，它们变得极其受欢迎。LMCOs通常通过将最终受体分子氧（O）的四电子还原与水相连来氧化各种底物。与漆酶相关的多铜氧化酶作为多基因家族广泛分布于高等植物的基因组序列中。本研究全面调查了甘蓝型油菜中的LMCO基因家族（Br-Lac）及其在各种非生物胁迫下的表达模式。共鉴定出位于五条不同染色体上的18个Br-Lac基因家族成员。系统发育分析将已记录的Br-Lac基因分为七组：第一组（四个基因）、第二组（九个基因）、第三组（八个基因）、第四组（四个基因）、第五组（六个基因）以及第六组和第七组（各一个基因）。基因结构和响应基序的关键特征在同一组内最为相似。此外，分歧研究还评估了Br-Lac基因的进化特征。预期的分歧时间范围为12365至39250万年前。我们还使用定量实时qRT-PCR确定了LMCO（Br-lac）基因家族18个已记录成员的关键作用。Br-Lac-6、Br-Lac-7、Br-Lac-8、Br-Lac-16、Br-Lac-17和Br-Lac-22对非生物胁迫（即干旱、高温和盐度）有积极响应。这些发现为甘蓝型油菜中LMCO基因的功能多样性奠定了基础。
