葡萄（Vitis vinifera）全基因组镁转运体基因家族的鉴定与表达分析。

BACKGROUND

Magnesium ion is one of the essential mineral elements for plant growth and development, which participates in a variety of physiological and biochemical processes. Since there is no report on the research of magnesium ion transporter in grape, the study of the structure and function of magnesium ion transporters (MGT) is helpful to understand the dynamic balance mechanism of intracellular magnesium ions and their inter- or intra-cellular activities.

RESULT

In this study, we identified the members of MGT protein family in grape and performed the phylogenetic and expression analysis. We have identified nine VvMGT genes in grape genome, which are distributed on eight different chromosomes. Phylogenetic analysis showed that MGT family members of grapes were divided into five subfamilies and had obvious homology with Arabidopsis, maize, and pear. Based on transcriptome data from the web databases, we analyzed the expression patterns of VvMGTs at different development stages and in response to abiotic stresses including waterlogging, drought, salinity, and copper. Using qRT-PCR method, we tested the expression of grape VvMGTs under magnesium and aluminum treatments and found significant changes in VvMGTs expression. In addition, four of the MGT proteins in grape were located in the nucleus.

CONCLUSION

Overall, in this study we investigated the structural characteristics, evolution pattern, and expression analysis of VvMGTs in depth, which laid the foundation for further revealing the function of VvMGT genes in grape.

背景

镁离子是植物生长和发育所必需的基本矿物质元素之一，它参与了多种生理和生化过程。由于目前尚未有关于葡萄镁离子转运蛋白的研究报道，因此研究镁离子转运蛋白（MGT）的结构和功能有助于了解细胞内镁离子的动态平衡机制及其在细胞内外的活动。

结果

在本研究中，我们鉴定了葡萄 MGT 蛋白家族的成员，并进行了系统发育和表达分析。我们在葡萄基因组中鉴定出 9 个 VvMGT 基因，它们分布在 8 条不同的染色体上。系统发育分析表明，葡萄 MGT 家族成员分为 5 个亚家族，与拟南芥、玉米和梨具有明显的同源性。基于网络数据库中的转录组数据，我们分析了 VvMGTs 在不同发育阶段和应对非生物胁迫（包括水淹、干旱、盐胁迫和铜胁迫）时的表达模式。利用 qRT-PCR 方法，我们检测了葡萄 VvMGTs 在镁和铝处理下的表达情况，发现 VvMGTs 的表达发生了显著变化。此外，葡萄中有 4 种 MGT 蛋白定位于细胞核中。