Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou, Hainan 570228, China.
Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, Hainan, China.
Sci Total Environ. 2022 Jul 15;830:154799. doi: 10.1016/j.scitotenv.2022.154799. Epub 2022 Mar 24.
The environmental accumulation of thiamethoxam has increasingly become a risk for the health of aquatic animals, especially crustacean species in the same phylum as the target pests. The lack of knowledge on the toxicity of thiamethoxam to crustaceans motivates our research to study the acute and chronic toxicity of decapod crustaceans Litopenaeus vannamei, exposed to thiamethoxam. A 28-day chronic toxicity test followed a 96 h acute toxicity test. Thiamethoxam induced oxidative stress and decreased growth performance in shrimp. In addition, thiamethoxam has led to a substantial imbalance of the micro-ecosystem in the intestine. The composition of the intestinal flora changed significantly, and the balance of the interaction network in genera was broken. The competitive interaction of many bacteria becomes an unstable cooperative interaction. Transcriptomic analysis showed that the numbers of up- and down-regulated differentially expressed genes (DEGs) increased in a dose-dependent manner. These DEGs were significantly enriched in pathways related to detoxification, and the expression of most detoxification genes was upregulated. DEGs related to detoxification were positively correlated with Shimia and negatively correlated with Pseudoalteromonas. This study provides evidence for the first time on the toxic effects of thiamethoxam on the growth, biochemistry, intestinal flora, and transcriptome in crustaceans.
噻虫嗪在环境中的积累对水生动物的健康，尤其是与靶标害虫同属一纲的甲壳类动物的健康，构成的威胁与日俱增。由于缺乏关于噻虫嗪对甲壳类动物毒性的知识，促使我们研究了暴露于噻虫嗪的十足目甲壳类动物凡纳滨对虾的急性和慢性毒性。在进行 28 天慢性毒性试验之前，先进行了 96 h 急性毒性试验。噻虫嗪导致虾类的氧化应激和生长性能下降。此外，噻虫嗪导致肠道微生态系统严重失衡。肠道菌群的组成发生了显著变化，属间互作网络的平衡被打破。许多细菌的竞争互作变成了不稳定的合作互作。转录组分析表明，上调和下调差异表达基因（DEGs）的数量呈剂量依赖性增加。这些 DEGs 显著富集在与解毒相关的途径中，大多数解毒基因的表达上调。与解毒相关的 DEGs 与 Shimia 呈正相关，与假交替单胞菌呈负相关。本研究首次为噻虫嗪对甲壳类动物的生长、生化、肠道菌群和转录组的毒性作用提供了证据。
