Trouvelot Sophie, Lemaitre-Guillier Christelle, Vallet Julie, Jacquens Lucile, Douillet Antonin, Harir Mourad, Larignon Philippe, Roullier-Gall Chloé, Schmitt-Kopplin Philippe, Adrian Marielle, Fontaine Florence
Agroécologie, Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Institut Agro Dijon, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
Université de Reims Champagne-Ardenne, Unité de recherche Résistance Induite et Bioprotection des Plantes (RIBP) USC Institut National de Recherche pour l'agriculture, l'alimentation et l'environnement (INRAE) 1488, Reims, France.
Front Plant Sci. 2023 Apr 11;14:1141700. doi: 10.3389/fpls.2023.1141700. eCollection 2023.
In the past, most grapevine trunk diseases (GTDs) have been controlled by treatments with sodium arsenite. For obvious reasons, sodium arsenite was banned in vineyards, and consequently, the management of GTDs is difficult due to the lack of methods with similar effectiveness. Sodium arsenite is known to have a fungicide effect and to affect the leaf physiology, but its effect on the woody tissues where the GTD pathogens are present is still poorly understood. This study thus focuses on the effect of sodium arsenite in woody tissues, particularly in the interaction area between asymptomatic wood and necrotic wood resulting from the GTD pathogens' activities. Metabolomics was used to obtain a metabolite fingerprint of sodium arsenite treatment and microscopy to visualize its effects at the histo-cytological level. The main results are that sodium arsenite impacts both metabolome and structural barriers in plant wood. We reported a stimulator effect on plant secondary metabolites in the wood, which add to its fungicide effect. Moreover, the pattern of some phytotoxins is affected, suggesting the possible effect of sodium arsenite in the pathogen metabolism and/or plant detoxification process. This study brings new elements to understanding the mode of action of sodium arsenite, which is useful in developing sustainable and eco-friendly strategies to better manage GTDs.
过去，大多数葡萄树干病害（GTDs）都通过亚砷酸钠处理来控制。由于显而易见的原因，葡萄园已禁止使用亚砷酸钠，因此，由于缺乏具有类似效果的方法，GTDs的管理变得困难。已知亚砷酸钠具有杀真菌作用并会影响叶片生理，但对存在GTD病原体的木质组织的影响仍知之甚少。因此，本研究聚焦于亚砷酸钠在木质组织中的作用，特别是在无症状木质与GTD病原体活动导致的坏死木质之间的相互作用区域。代谢组学用于获取亚砷酸钠处理的代谢物指纹图谱，显微镜技术用于在组织细胞学水平上可视化其作用效果。主要结果是亚砷酸钠会影响植物木材中的代谢组和结构屏障。我们报道了其对木材中植物次生代谢物的刺激作用，这增强了其杀真菌效果。此外，一些植物毒素的模式受到影响，这表明亚砷酸钠可能对病原体代谢和/或植物解毒过程产生作用。本研究为理解亚砷酸钠的作用方式带来了新的要素，这有助于制定可持续且环保的策略以更好地管理GTDs。
