Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Environ Res. 2023 Aug 15;231(Pt 2):116120. doi: 10.1016/j.envres.2023.116120. Epub 2023 May 12.
2,6-Di-tert-butyl-hydroxytotulene (BHT) is an additive commonly used in the manufacturing of lubricants to improve their antioxidant properties. However, in this study, we found that BHT affects the biodegradation of bio-lubricants by influencing the microbial community during the degradation of bio-lubricants. Specifically, BHT was found to reduce bacterial richness in activated sludge, but it increased the relative abundance of Actinobacteria (from 21.24% to 40.89%), Rhodococcus (from 17.15% to 31.25%), Dietzia (from 0.069% to 6.49%), and Aequorivita (from 0.90% to 1.85%). LEfSe analysis and co-occurrence network analysis suggested that Actinobacteria could be potential biomarkers and keystone taxa in microbial communities. Using the MetaCyc pathway database, the study found that BHT interfered with cellular biosynthetic processes. Additionally, the study also showed that mineral-lubricant base oils, which are difficult to degrade, significantly altered the diversity and composition of the microbiome. Overall, the findings demonstrate that BHT and mineral-lubricant base oils can substantially alter bacterial richness, structure, and function, potentially contributing to the difficulty in degrading lubricants. These findings have implications for the development of more biodegradable lubricants and the management of industrial waste containing lubricants.
2,6-二叔丁基对甲酚（BHT）是一种常用的添加剂，用于制造润滑剂，以提高其抗氧化性能。然而，在本研究中，我们发现 BHT 通过影响生物润滑剂降解过程中的微生物群落，影响生物润滑剂的生物降解。具体来说，BHT 被发现降低了活性污泥中的细菌丰富度，但增加了放线菌（从 21.24%增加到 40.89%）、红球菌（从 17.15%增加到 31.25%）、迪茨氏菌（从 0.069%增加到 6.49%）和海杆菌（从 0.90%增加到 1.85%）的相对丰度。LEfSe 分析和共现网络分析表明，放线菌可能是微生物群落中的潜在生物标志物和关键分类群。利用 MetaCyc 途径数据库，研究发现 BHT 干扰了细胞生物合成过程。此外，研究还表明，难以降解的矿物型基础油显著改变了微生物组的多样性和组成。总的来说，这些发现表明 BHT 和矿物型基础油可以显著改变细菌的丰富度、结构和功能，这可能导致润滑剂降解困难。这些发现对于开发更具生物降解性的润滑剂和管理含润滑剂的工业废物具有重要意义。
