Validation of resonance Raman spectroscopy-measured skin carotenoid status as a biomarker for fruit and vegetable intake in Korean adults.

Blood carotenoid concentration measurement is considered the gold standard for fruit and vegetable (F&V) intake estimation; however, this method is invasive and expensive. Recently, skin carotenoid status (SCS) measured by optical sensors has been evaluated as a promising parameter for F&V intake estimation. In this cross-sectional study, we aimed to validate the utility of resonance Raman spectroscopy (RRS)-assessed SCS as a biomarker of F&V intake in Korean adults. We used data from 108 participants aged 20-69 years who completed SCS measurements, blood collection and 3-d dietary recordings. Serum carotenoid concentrations were quantified using HPLC, and dietary carotenoid and F&V intakes were estimated via 3-d dietary records using a carotenoid database for common Korean foods. The correlations of the SCS with serum carotenoid concentrations, dietary carotenoid intake and F&V intake were examined to assess SCS validity. SCS was positively correlated with total serum carotenoid concentration ( = 0·52, 95 % CI = 0·36, 0·64, < 0·001), serum -carotene concentration ( = 0·60, 95 % CI = 0·47, 0·71, < 0·001), total carotenoid intake ( = 0·20, 95 % CI = 0·01, 0·37, = 0·04), -carotene intake ( = 0·30, 95 % CI = 0·11, 0·46, = 0·002) and F&V intake ( = 0·40, 95 % CI = 0·23, 0·55, < 0·001). These results suggest that SCS can be a valid biomarker of F&V intake in Korean adults.