来自大鼠心脏的快速形成的寡聚四磷酸腺苷衍生物的分子结构。

The molecular structure of a rapidly formed oligomeric adenosine tetraphosphate derivative from rat heart.

作者信息

Hutchinson W L, Morris P G, Mowbray J

出版信息

Biochem J. 1986 Mar 15;234(3):623-7. doi: 10.1042/bj2340623.

DOI:10.1042/bj2340623

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146617/

Abstract

The inability to account for large systematic variations with time in soluble adenine nucleotides in perfused rat hearts [Bates, Perrett & Mowbray (1978) Biochem. J. 176, 485-493; Mowbray, Bates & Perrett (1981) FEBS Lett. 131, 55-59; Mowbray, Perrett & Bates (1984) Int. J. Biochem. 16, 889-894] led us to show that the soluble nucleotides are in rapid equilibrium with some hitherto unrecognized trichloroacetic acid/methanol-precipitable highly phosphorylated heteropolymeric form [Mowbray, Hutchinson, Tibbs & Morris (1984) Biochem. J. 223, 627-632]. Selective digestion coupled to chromatographic analysis together with m.s. and 31P-n.m.r. spectrometry have now been used to show that the likely structure for a purified oligomer that is in specific-radioactivity equilibrium with tissue ATP is 3-phospho-[glyceroyl-gamma-triphosphoroyl-5'-adenosine-3'-3- phospho]4 glyceroyl-gamma-triphosphoroyl-5'-adenosine.

摘要

在灌注大鼠心脏中，可溶性腺嘌呤核苷酸随时间出现的较大系统性变化无法得到解释【贝茨、佩雷特和莫布雷（1978年）《生物化学杂志》第176卷，第485 - 493页；莫布雷、贝茨和佩雷特（1981年）《欧洲生物化学学会联合会快报》第131卷，第55 - 59页；莫布雷、佩雷特和贝茨（1984年）《国际生物化学杂志》第16卷，第889 - 894页】，这促使我们发现，可溶性核苷酸与某种迄今未被认识的三氯乙酸/甲醇可沉淀的高度磷酸化杂聚体形式处于快速平衡状态【莫布雷、哈钦森、蒂布斯和莫里斯（1984年）《生物化学杂志》第223卷，第627 - 632页】。现在，结合色谱分析的选择性消化以及质谱和³¹P - 核磁共振光谱已被用于表明，与组织ATP处于比放射性平衡的纯化寡聚体的可能结构是3 - 磷酸 - [甘油酰 - γ - 三磷酸酰基 - 5'-腺苷 - 3'-3 - 磷酸]4甘油酰 - γ - 三磷酸酰基 - 5'-腺苷。

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Studies on fetuin, a glycoprotein of fetal serum. I. Isolation, chemical composition, and physiochemical properties.关于胎球蛋白（一种胎儿血清糖蛋白）的研究。I. 分离、化学成分及理化性质。

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Separation of the phosphoric esters on the filter paper chromatogram.磷酸酯在滤纸色谱上的分离

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In perfused rat hearts ischaemia promotes the reversible conversion of appreciable quantities of soluble adenine nucleotides to a stable trichloroacetic acid-precipitable form.在灌注的大鼠心脏中，缺血促使相当数量的可溶性腺嘌呤核苷酸可逆地转化为一种稳定的可被三氯乙酸沉淀的形式。

Biosci Rep. 1981 Nov;1(11):857-63. doi: 10.1007/BF01114819.

5

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Inversely related oscillations in the contents of cyclic GMP and the total adenine nucleotides in steady-state perfused rat hearts.

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