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来自大鼠心脏的快速形成的寡聚四磷酸腺苷衍生物的分子结构。

The molecular structure of a rapidly formed oligomeric adenosine tetraphosphate derivative from rat heart.

作者信息

Hutchinson W L, Morris P G, Mowbray J

出版信息

Biochem J. 1986 Mar 15;234(3):623-7. doi: 10.1042/bj2340623.

DOI:10.1042/bj2340623
PMID:3718488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146617/
Abstract

The inability to account for large systematic variations with time in soluble adenine nucleotides in perfused rat hearts [Bates, Perrett & Mowbray (1978) Biochem. J. 176, 485-493; Mowbray, Bates & Perrett (1981) FEBS Lett. 131, 55-59; Mowbray, Perrett & Bates (1984) Int. J. Biochem. 16, 889-894] led us to show that the soluble nucleotides are in rapid equilibrium with some hitherto unrecognized trichloroacetic acid/methanol-precipitable highly phosphorylated heteropolymeric form [Mowbray, Hutchinson, Tibbs & Morris (1984) Biochem. J. 223, 627-632]. Selective digestion coupled to chromatographic analysis together with m.s. and 31P-n.m.r. spectrometry have now been used to show that the likely structure for a purified oligomer that is in specific-radioactivity equilibrium with tissue ATP is 3-phospho-[glyceroyl-gamma-triphosphoroyl-5'-adenosine-3'-3- phospho]4 glyceroyl-gamma-triphosphoroyl-5'-adenosine.

摘要

在灌注大鼠心脏中，可溶性腺嘌呤核苷酸随时间出现的较大系统性变化无法得到解释【贝茨、佩雷特和莫布雷（1978年）《生物化学杂志》第176卷，第485 - 493页；莫布雷、贝茨和佩雷特（1981年）《欧洲生物化学学会联合会快报》第131卷，第55 - 59页；莫布雷、佩雷特和贝茨（1984年）《国际生物化学杂志》第16卷，第889 - 894页】，这促使我们发现，可溶性核苷酸与某种迄今未被认识的三氯乙酸/甲醇可沉淀的高度磷酸化杂聚体形式处于快速平衡状态【莫布雷、哈钦森、蒂布斯和莫里斯（1984年）《生物化学杂志》第223卷，第627 - 632页】。现在，结合色谱分析的选择性消化以及质谱和³¹P - 核磁共振光谱已被用于表明，与组织ATP处于比放射性平衡的纯化寡聚体的可能结构是3 - 磷酸 - [甘油酰 - γ - 三磷酸酰基 - 5'-腺苷 - 3'-3 - 磷酸]4甘油酰 - γ - 三磷酸酰基 - 5'-腺苷。

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