Hutchinson W L, Bates D J, Perrett D, Mowbray J
Biosci Rep. 1981 Nov;1(11):857-63. doi: 10.1007/BF01114819.
Radioactivity from [14C] adenosine was linearly incorporated into tissue nucleotides in perfused rat hearts. All the TCA-extractable 14C was confined to the purine nucleoside phosphates for up to 1 h of perfusion. Radioactivity was also incorporated linearly into the TCA-insoluble fraction, which by 40 min accounted for 24% of the tissue 14 C. Estimates based on precursor specific radioactivity suggest that at least 0.6 micro mol/g of the mononucleotide is in this stable insoluble form. Following 2 min total ischaemia, the tissue nucleotide content and soluble radioactivity decreased while the insoluble radioactivity showed a corresponding increase to account now for 35% of the tissue radiolabel. This redistribution was rapidly reversed by post-ischaemic reperfusion. A possible function for the rapid reversible sequestration of adenine nucleotides in ischaemia is proposed.
[14C]腺苷的放射性被线性整合到灌注大鼠心脏的组织核苷酸中。在长达1小时的灌注过程中，所有三氯乙酸（TCA）可提取的14C都局限于嘌呤核苷磷酸中。放射性也被线性整合到TCA不溶性部分，到40分钟时，该部分占组织14C的24%。基于前体比放射性的估计表明，至少0.6微摩尔/克的单核苷酸处于这种稳定的不溶性形式。在2分钟的完全缺血后，组织核苷酸含量和可溶性放射性降低，而不溶性放射性相应增加，现在占组织放射性标记的35%。这种重新分布在缺血后再灌注时迅速逆转。本文提出了腺嘌呤核苷酸在缺血时快速可逆隔离的一种可能功能。
