采用安培检测法对尿卟啉原和粪卟啉原异构体进行高效液相色谱分析。
High-performance liquid chromatography of uroporphyrinogen and coproporphyrinogen isomers with amperometric detection.
作者信息
Lim C K, Li F, Peters T J
出版信息
Biochem J. 1986 Mar 15;234(3):629-33. doi: 10.1042/bj2340629.
Abstract
A reversed-phase h.p.l.c. system, with an ODS-Hypersil column with acetonitrile or methanol in ammonium acetate buffer as mobile phase, is described for the separation of uro-and copro-porphyrinogen isomers. The porphyrinogens are detected amperometrically with sensitivity comparable with that of the fluorescent detection of porphyrins. The effects of pH, buffer concentration and organic modifiers on retention and resolution were studied. The method is suitable for both analytical and preparative separation of porphyrinogens.
摘要
本文描述了一种反相高效液相色谱系统，该系统采用ODS-Hypersil柱，以乙腈或甲醇与醋酸铵缓冲液作为流动相，用于分离尿卟啉原和粪卟啉原异构体。通过安培检测法对卟啉原进行检测，其灵敏度与卟啉的荧光检测相当。研究了pH值、缓冲液浓度和有机改性剂对保留时间和分离度的影响。该方法适用于卟啉原的分析分离和制备分离。
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引用本文的文献
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本文引用的文献
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Diagnosis of porphyrias by ion-pair high-performance liquid chromatography.
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2
High-performance liquid chromatography of coproporphyrin isomers.粪卟啉异构体的高效液相色谱法
Biochem J. 1983 Feb 1;209(2):553-5. doi: 10.1042/bj2090553.
3
Urine and faecal porphyrin profiles by reversed-phase high-performance liquid chromatography in the porphyrias.通过反相高效液相色谱法测定卟啉病患者尿液和粪便中的卟啉谱。
Clin Chim Acta. 1984 May 16;139(1):55-63. doi: 10.1016/0009-8981(84)90192-x.
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High-performance liquid chromatography of naturally occurring 8-, 7-, 6-, 5- and 4-carboxylic porphyrin isomers.天然存在的8-、7-、6-、5-和4-羧基卟啉异构体的高效液相色谱分析
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