Lim C K, Li F, Peters T J
Biochem J. 1986 Mar 15;234(3):629-33. doi: 10.1042/bj2340629.
A reversed-phase h.p.l.c. system, with an ODS-Hypersil column with acetonitrile or methanol in ammonium acetate buffer as mobile phase, is described for the separation of uro-and copro-porphyrinogen isomers. The porphyrinogens are detected amperometrically with sensitivity comparable with that of the fluorescent detection of porphyrins. The effects of pH, buffer concentration and organic modifiers on retention and resolution were studied. The method is suitable for both analytical and preparative separation of porphyrinogens.
本文描述了一种反相高效液相色谱系统，该系统采用ODS-Hypersil柱，以乙腈或甲醇与醋酸铵缓冲液作为流动相，用于分离尿卟啉原和粪卟啉原异构体。通过安培检测法对卟啉原进行检测，其灵敏度与卟啉的荧光检测相当。研究了pH值、缓冲液浓度和有机改性剂对保留时间和分离度的影响。该方法适用于卟啉原的分析分离和制备分离。
