新种新种新种和新种，从亚热带森林土壤中分离得到。

sp. nov. sp. nov. sp. nov. and sp. nov., isolated from subtropical forest soil.

作者信息

Feng Guang-Da, Deng Xiaoqin, Li Jiali, Chen Wendi, Zhang Xianjiao, Zhu Honghui

机构信息

Key Laboratory of Agricultural Microbiomics and Precision Application (MARA); Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application; Key Laboratory of Agricultural Microbiome (MARA); State Key Laboratory of Applied Microbiology Southern China; Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, PR China.

出版信息

Int J Syst Evol Microbiol. 2023 May;73(5). doi: 10.1099/ijsem.0.005878.

DOI:10.1099/ijsem.0.005878

PMID:37185089

Abstract

Four novel bacterial strains, designated RBB1W86, RXD159, RBB189 and RLT163, were isolated from subtropical forest soil of the Nanling National Nature Reserve located in Guangdong Province, PR China. 16S rRNA gene phylogeny indicated their affiliation to the genus , among which strains RBB1W86 and RXD159 were closely related to CGMCC 1.15435 with 16S rRNA gene sequence similarities of 98.8 and 99.5 %, respectively, and strains RBB189 and RLT163 were closely related to CGMCC 1.16273 (98.8 %) and JCM 21530 (99.4 %), respectively. Phylogenomic analysis based on 92 core genes showed consistent phylogeny with the 16S rRNA gene phylogeny for strains RBB1W86, RBB189 and RLT163, while strain RXD159 showed a closer relationship with CGMCC 1.16273 and strain RBB189. The genome-derived average nucleotide identity (ANI) values between the newly isolated strains and their closely related species were 70.18‒90.20 %, and the corresponding digital DNA-DNA hybridization (dDDH) values were 20.80‒40.30 %. Meanwhile, the ANI and dDDH values between each pair of the newly isolated strains were 75.80‒79.77 % and 21.30‒23.30 %, respectively. They all took iso-C and summed feature 9 (10-methyl C and/or iso-C 9) as the major fatty acids. Moreover, C, iso-C, iso-C and summed feature 3 (C 7 and/or C 6) were also variously distributed as major components. They all took ubiquinone 8 as the only predominant respiratory quinone and phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as the major polar lipids. Phosphatidylmethylethanolamine was only present in strain RBB189 as another major component. Based on the results of phenotypic, genotypic and chemotaxonomic analyses, the newly isolated strains could be clearly distinguished from their closely related species and should represent four distinct novel species of the genus , for which the names sp. nov. (type strain RBB1W86=GDMCC 1.1901=KACC 21988), sp. nov. (type strain RXD159=GDMCC 1.1902=KACC 21989), sp. nov. (type strain RBB189=GDMCC 1.1900=KACC 21990 ) and sp. nov. (type strain RLT163=GDMCC 1.1916=KACC 21991) are proposed.

摘要

从中国广东省南岭国家级自然保护区的亚热带森林土壤中分离出四株新的细菌菌株，分别命名为RBB1W86、RXD159、RBB189和RLT163。16S rRNA基因系统发育分析表明它们隶属于属，其中菌株RBB1W86和RXD159分别与CGMCC 1.15435密切相关，16S rRNA基因序列相似性分别为98.8%和99.5%，菌株RBB189和RLT163分别与CGMCC 1.16273（98.8%）和JCM 21530（99.4%）密切相关。基于92个核心基因的系统基因组分析显示，菌株RBB1W86、RBB189和RLT163的系统发育与16S rRNA基因系统发育一致，而菌株RXD159与CGMCC 1.16273和菌株RBB189的关系更密切。新分离菌株与其密切相关物种之间的基因组衍生平均核苷酸同一性（ANI）值为70.18 - 90.20%，相应的数字DNA - DNA杂交（dDDH）值为20.80 - 40.30%。同时，新分离菌株两两之间的ANI和dDDH值分别为75.80 - 79.77%和21.30 - 23.30%。它们均以异-C和总和特征9（10-甲基-C和/或异-C9）作为主要脂肪酸。此外，C、异-C、异-C和总和特征3（C7和/或C6）也作为主要成分呈不同分布。它们均以泛醌8作为唯一主要的呼吸醌，以磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂作为主要极性脂质。磷脂酰甲基乙醇胺仅作为另一主要成分存在于菌株RBB189中。基于表型、基因型和化学分类分析结果，新分离菌株可与它们的密切相关物种明显区分，应代表属的四个不同新物种，为此提出新物种名称新种（模式菌株RBB1W86 = GDMCC 1.1901 = KACC 21988）、新种（模式菌株RXD159 = GDMCC 1.1902 = KACC 21989）、新种（模式菌株RBB189 = GDMCC 1.1900 = KACC 21990）和新种（模式菌株RLT163 = GDMCC 1.1916 = KACC 21991）。