Kątnik Ewa, Gomułkiewicz Agnieszka, Piotrowska Aleksandra, Grzegrzółka Jędrzej, Rusak Agnieszka, Kmiecik Alicja, Ratajczak-Wielgomas Katarzyna, Dzięgiel Piotr
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Curr Issues Mol Biol. 2023 Mar 23;45(4):2681-2698. doi: 10.3390/cimb45040175.
B-cell leukemia/lymphoma 11A (BCL11A) is a transcription factor that regulates the expression of genes involved in cell division or apoptosis. A link between high BCL11A expression and a worse prognosis has been demonstrated in patients with various cancers. The aim of this study was to investigate the expression pattern of BCL11A in breast cancer (BC) cases and mastopathy samples and to correlate the results with the clinicopathological data. The expression of the BCL11A protein was investigated using immunohistochemistry (IHC) on 200 cases of BC and 13 mastopathy samples. The level of BCL11A mRNA was determined using real-time PCR in 22 cases of BC and 6 mastopathy samples. The expression of BCL11A was also examined at the protein and mRNA levels in BC cell lines. A higher expression level of BCL11A in BC cases was shown compared to mastopathy samples. The expression level of BCL11A in BC cases and in the studied cell lines decreased with the increasing grade of histological malignancy (G). It was also negatively correlated with the primary tumor size. A significantly lower expression of BCL11A was found in BC that did not express estrogen or progesterone receptors and in triple-negative cases. The results of our research suggest that BCL11A may be relevant in the development of BC.
B细胞白血病/淋巴瘤11A（BCL11A）是一种转录因子，可调节参与细胞分裂或细胞凋亡的基因的表达。在各种癌症患者中，已证实BCL11A高表达与较差的预后之间存在关联。本研究的目的是调查BCL11A在乳腺癌（BC）病例和乳腺病样本中的表达模式，并将结果与临床病理数据相关联。使用免疫组织化学（IHC）对200例BC病例和13例乳腺病样本进行BCL11A蛋白表达的研究。使用实时PCR在22例BC病例和6例乳腺病样本中测定BCL11A mRNA水平。还在BC细胞系中在蛋白质和mRNA水平上检测BCL11A的表达。与乳腺病样本相比，BC病例中BCL11A的表达水平更高。BC病例和所研究细胞系中BCL11A的表达水平随着组织学恶性程度（G）的增加而降低。它也与原发肿瘤大小呈负相关。在不表达雌激素或孕激素受体的BC以及三阴性病例中发现BCL11A的表达明显较低。我们的研究结果表明，BCL11A可能与BC的发生发展相关。
