Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Department of Biochemistry and Molecular Biology, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Int J Mol Sci. 2023 Jun 7;24(12):9848. doi: 10.3390/ijms24129848.
B-cell leukemia/lymphoma 11A (BCL11A) may be one of the potential biomarkers of non-small cell lung cancer (NSCLC). However, its role in the development of this cancer has not yet been precisely established. The aim of this study was to investigate the expression of BCL11A at the mRNA and protein levels in NSCLC cases and non-malignant lung tissue (NMLT) and to determine the relationship between BCL11A expression and the clinicopathological factors and Ki-67, Slug, Snail and Twist. The localization and the level of BCL11A protein were examined using immunohistochemistry (IHC) on 259 cases of NSCLC, and 116 NMLT samples were prepared as tissue microarrays and using immunofluorescence (IF) in the following cell lines: NCI-H1703, A549 and IMR-90. The mRNA expression of BCL11A was determined using real-time PCR in 33 NSCLC cases, 10 NMLT samples and the cell lines. BCL11A protein expression was significantly higher in NSCLC cases compared to NMLT. Nuclear expression was found in lung squamous cell carcinoma (SCC) cells, while cytoplasmic expression was demonstrated in adenocarcinoma (AC) cells. Nuclear expression of BCL11A decreased with increasing malignancy grade and correlated positively with Ki-67 and Slug and Twist expression. The opposite relationships were found for the cytoplasmic expression of BCL11A. Nuclear expression of BCL11A in NSCLC cells may affect tumor cell proliferation and change their phenotype, thus promoting tumor progression.
B 细胞白血病/淋巴瘤 11A（BCL11A）可能是一种非小细胞肺癌（NSCLC）的潜在生物标志物。然而，其在这种癌症发展中的作用尚未被精确确定。本研究旨在调查 BCL11A 在 NSCLC 病例和非恶性肺组织（NMLT）中的 mRNA 和蛋白水平的表达，并确定 BCL11A 表达与临床病理因素以及 Ki-67、Slug、Snail 和 Twist 之间的关系。使用免疫组织化学（IHC）检查了 259 例 NSCLC 病例和 116 例 NMLT 样本的 BCL11A 蛋白的定位和水平，并使用免疫荧光（IF）在以下细胞系中进行：NCI-H1703、A549 和 IMR-90。使用实时 PCR 确定了 33 例 NSCLC 病例、10 例 NMLT 样本和细胞系中 BCL11A 的 mRNA 表达。与 NMLT 相比，BCL11A 蛋白在 NSCLC 病例中表达明显更高。核表达见于肺鳞状细胞癌（SCC）细胞，而细胞质表达见于腺癌（AC）细胞。BCL11A 的核表达随着恶性程度的增加而降低，与 Ki-67 和 Slug 和 Twist 的表达呈正相关。BCL11A 的细胞质表达则相反。BCL11A 在 NSCLC 细胞中的核表达可能影响肿瘤细胞的增殖并改变其表型，从而促进肿瘤的进展。
Zotero 插件
