Foa P, Maiolo A T, Baldini L, Quarto di Palo F, Starace G, Polli E
Boll Ist Sieroter Milan. 1986;65(1):65-8.
Cyclosporin A (CyA) is a potent immunosuppressive agent which has been reported to inhibit proliferation of human T leukemic lymphoblasts. We used the permanent cell line 8402 to perform a detailed investigation of the CyA mode of action on such cells. CyA treatment resulted in a dose-dependent inhibition of the 8402 cells growth rate which was paralleled by a decrease of tritiated thymidine uptake. These findings were the consequence of redistribution of treated cells in the cell cycle phase. By means of intracellular DNA content measurements made by flow cytometry, it was shown that the primary effect of CyA was accumulation of treated cells at the G1 phase of the cell cycle. At the doses used, CyA activity was not spontaneously reversible, resulting in death of treated cells which was apparent at about 72 h after exposure to the drug.
环孢素A（CyA）是一种强效免疫抑制剂，据报道它能抑制人T白血病淋巴母细胞的增殖。我们使用永久细胞系8402对CyA作用于此类细胞的作用模式进行了详细研究。CyA处理导致8402细胞生长速率呈剂量依赖性抑制，同时氚标记胸腺嘧啶核苷摄取量减少。这些发现是处理后的细胞在细胞周期阶段重新分布的结果。通过流式细胞术测量细胞内DNA含量表明，CyA的主要作用是使处理后的细胞在细胞周期的G1期积累。在所使用的剂量下，CyA的活性不会自发逆转，导致处理后的细胞死亡，这在接触该药物约72小时后明显可见。
