Xu Jianmin, Yan Zhiming, Xu Zhigang, Wang Yuanhua, Xie Zhenqiang
1College of Horticulture, Nanjing Agricultural University, Nanjing, 210095 China.
Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400 China.
3 Biotech. 2018 Sep;8(9):394. doi: 10.1007/s13205-018-1410-0. Epub 2018 Sep 1.
Light is an important factor for plant development and has serious effects on the growth, production and quality of potatoes. However, the physical and molecular mechanisms by which potato plantlets cope with different light qualities are not understood. In this study, the potato "Zhuanxinwu", which is a germplasm potato resource with a high anthocyanin content, was used for physiological and transcriptome profiling analyses to uncover the different mechanisms that occur in response to blue, red and white light conditions, with the white light condition serving as the control. Multiple growth indexes, protective enzyme activity and metabolite accumulation were measured. The results indicated that white light promoted a shift in biomass allocation away from tubers to leaves to enhance dry leaf matter and reduce tuber fresh/dry weight relative to the effects of blue or red light. The leaf area and anthocyanin content values were greater for plants grown in blue light than those grown in white or red light, suggesting that combinations of different spectra were more conducive to regulating potato growth. A total of 2220 differentially expressed genes (DEGs) were found among the three samples, and the DEGs in the three comparison sets were analyzed. A total of 1180 and 984 DEGs were identified in the red light (Red) and blue light (Blue) conditions compared to the control condition, respectively, and 359 DEGs overlapped between the two comparison sets (Blue_vs_White and Red_vs_White). Interestingly, the 24 most common overlapped DEGs were involved in photosynthesis, respiration, and reactive oxygen species (ROS) scavenging. Of these DEGs, four genes involved in photosynthesis and two genes involved in pigment synthesis were highly expressed, implying that some genes could be implemented to cope with different light spectra by regulating the expression of DEGs involved in the corresponding metabolic pathways. In conclusion, our study characterizes physiological responses of potato to different light qualities and identifies potential pathways and candidate genes involved in these responses, thus providing a basis for further research on artificial light regulation of potato plant growth.
光是植物发育的重要因素，对马铃薯的生长、产量和品质有严重影响。然而，马铃薯幼苗应对不同光质的物理和分子机制尚不清楚。在本研究中，以花青素含量高的种质马铃薯资源“转心乌”为材料，进行生理和转录组分析，以揭示其在蓝光、红光和白光条件下（以白光为对照）所发生的不同机制。测定了多个生长指标、保护酶活性和代谢物积累。结果表明，与蓝光或红光相比，白光促进了生物量分配从块茎向叶片的转移，从而增加了叶片干物质，降低了块茎鲜重/干重。蓝光下生长的植株叶面积和花青素含量值高于白光或红光下生长的植株，这表明不同光谱组合更有利于调控马铃薯生长。在三个样本中共发现2220个差异表达基因（DEG），并对三个比较组中的DEG进行了分析。与对照条件相比，红光（Red）和蓝光（Blue）条件下分别鉴定出1180个和984个DEG，两个比较组（Blue_vs_White和Red_vs_White）之间有359个DEG重叠。有趣的是，24个最常见的重叠DEG参与光合作用、呼吸作用和活性氧（ROS）清除。在这些DEG中，四个参与光合作用的基因和两个参与色素合成的基因高表达，这意味着一些基因可以通过调控相应代谢途径中DEG的表达来应对不同光谱。总之，我们的研究表征了马铃薯对不同光质的生理反应，并鉴定了参与这些反应的潜在途径和候选基因，从而为进一步研究人工光调控马铃薯植株生长提供了依据。
