Alghamdi Amira M, Al-Abbasi Fahad A, AlGhamdi Shareefa A, Fatima Farhat, Alzarea Sami I, Kazmi Imran
Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Saudi J Biol Sci. 2023 Jun;30(6):103656. doi: 10.1016/j.sjbs.2023.103656. Epub 2023 Apr 23.
The examination was sighted to study the preventive effects of rosinidin against rotenone-activated Parkinson's disease in rats.
Animals were randamoized into five groups: I-saline, II-rotenone (0.5 mg/kg/b.wt.), III- IV-10 and 20 mg/kg rosinidin after rotenone and V-20 mg/kg rosinidin per se for 28 days and were assigned for behavioral analysis., Biochemical parameters i.e. lipid peroxidation, endogenous antioxidants, nitrite level, neurotransmitter levels, proinflammatory biomarkers such as interleukin- 6 (IL-6), tumor necrosis factor-α, IL-1β, nuclear factor kappa B, nuclear factor erythroid 2-related factor 2, and caspase-3 were assessed on the 29th day of the research.
Rosinidin augmented the effectiveness of rotenone on akinesia, catalepsy, forced-swim test, rotarod, and open-field test. Biochemical findings indicated that treatment of rosinidin showed restoring neuroinflammatory cytokines, antioxidants, and neurotransmitter levels in rotenone-injected rats.
As a result of rosinidin treatment, the brain was protected from oxidative stress-induced neuronal damage and inhibited neuroinflammatory cytokines.
本实验旨在研究罗西尼定对鱼藤酮诱导的大鼠帕金森病的预防作用。
将动物随机分为五组：I组-生理盐水组，II组-鱼藤酮组（0.5毫克/千克/体重），III组-IV组-鱼藤酮注射后分别给予10毫克/千克和20毫克/千克罗西尼定，V组-单独给予20毫克/千克罗西尼定，持续28天，并进行行为分析。在研究的第29天评估生化参数，即脂质过氧化、内源性抗氧化剂、亚硝酸盐水平、神经递质水平、促炎生物标志物如白细胞介素-6（IL-6）、肿瘤坏死因子-α、IL-1β、核因子κB、核因子红细胞2相关因子2和半胱天冬酶-3。
罗西尼定增强了鱼藤酮对运动不能、僵住症、强迫游泳试验、转棒试验和旷场试验的作用效果。生化结果表明，罗西尼定治疗可使注射鱼藤酮的大鼠的神经炎症细胞因子、抗氧化剂和神经递质水平恢复正常。
罗西尼定治疗可保护大脑免受氧化应激诱导的神经元损伤，并抑制神经炎症细胞因子。
