Odoh C K, Kamal R, Xue H, Lyu L, Arnone J T, Zhao Z K
Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Rd, Dalian, 116023 China.
University of Chinese Academy of Sciences, Beijing, 100049 China.
Indian J Microbiol. 2023 Mar;63(1):42-49. doi: 10.1007/s12088-023-01055-y. Epub 2023 Jan 9.
Glucosylglycerol (GG) is an osmolyte that protects cells from extreme conditions. It is produced by sucrose phosphorylase, an enzyme that uses sucrose and glycerol as substrate. GG protects tissue integrity in desert plants during harsh conditions and guards cyanobacteria against high salinity (halotolerant). However, no extensive research has been conducted on the lifespan application of this compound on the yeast . We designed this study to (1) characterize GG's effect on yeast chronological lifespan (CLS) and (2) to determine the mechanisms underlying its lifespan promotion on strain DBY746. The results obtained in our study confirm that GG causes increased longevity when administered at moderate doses (48 mM and 120 mM). In addition, we discovered that GG promotes yeast cell longevity by increasing the osmolarity of the culture medium. The maximum lifespan increased by approximately 15.38% and 34.6%, (i.e., 115.38 and 134.61) respectively, upon administration of GG at 48 mM and 120 mM concentrations. Elucidation of the mechanisms underlying this positive response suggests that GG promotes CLS by activities that modulate reactive oxygen species (ROS) generation, as evident in its increased ROS generation (mitohormesis). An increase in medium osmolarity caused by GG supplementation triggers ROS production and promotes longevity in the yeast (. An in-depth study on the potential application of this molecule in aging research is crucial; this will aid in expounding the mechanisms of this geroprotector and its longevity supportive tendencies.
The online version contains supplementary material available at 10.1007/s12088-023-01055-y.
葡糖基甘油（GG）是一种能保护细胞免受极端条件影响的渗透调节物质。它由蔗糖磷酸化酶产生，该酶以蔗糖和甘油为底物。GG在恶劣条件下保护沙漠植物的组织完整性，并保护蓝细菌免受高盐度影响（耐盐）。然而，关于这种化合物在酵母寿命方面的应用尚未进行广泛研究。我们设计了本研究以（1）表征GG对酵母时序寿命（CLS）的影响，以及（2）确定其对DBY746菌株寿命促进作用的潜在机制。我们研究获得的结果证实，以中等剂量（48 mM和120 mM）施用GG会导致寿命延长。此外，我们发现GG通过提高培养基的渗透压来促进酵母细胞寿命。在分别以48 mM和120 mM浓度施用GG后，最大寿命分别增加了约15.38%和34.6%（即115.38和134.61）。对这种积极反应潜在机制的阐释表明，GG通过调节活性氧（ROS）生成的活动促进CLS，这在其增加的ROS生成（线粒体应激）中很明显。GG添加导致的培养基渗透压增加触发了ROS产生并促进了酵母的寿命。深入研究该分子在衰老研究中的潜在应用至关重要；这将有助于阐明这种老年保护剂的机制及其寿命支持趋势。
在线版本包含可在10.1007/s12088 - 023 - 01055 - y获取的补充材料。
