Turner D R, Wilson D M, Lake A, Heaton J M, Leibowitz S, Cameron J S
Clin Nephrol. 1979 Jan;11(1):13-7.
An evaluation of the use of horseradish peroxidase labelled antisera against IgG, IgM, IgA and C3 compared with corresponding antisera labelled with fluorescein isothiocyanate has been carried out on renal biopsy material from 200 patients with renal disease. A disparity between the two methods was observed in 10-19% of the readings according to the antigen being demonstrated. The majority of differences recorded would not have influenced the executive diagnosis. Where the immunological results could have influenced the executive diagnosis, 5 probable false results were obtained using innumofluorescence and 4 with immunoperoxidase. Immunoperoxidase has the advantage over immunofluorescence in that it provides a permanent preparation which can be reviewed months or years later. In addition it provides more accurate localization of the immune deposits since the section can be counterstained with Mayer's hemalum and viewed by conventional light microscopy. In view of these advantages we recommend that serious consideration should be given to using immunoperoxidase in place of immunofluorescence for the examination of renal biopsy material.
对200例肾病患者的肾活检材料进行了一项评估，比较了辣根过氧化物酶标记的抗IgG、IgM、IgA和C3抗血清与异硫氰酸荧光素标记的相应抗血清的使用情况。根据所检测的抗原不同，两种方法在10%-19%的读数中存在差异。记录的大多数差异不会影响最终诊断。在免疫结果可能影响最终诊断的情况下，免疫荧光法得到了5个可能的假结果，免疫过氧化物酶法得到了4个。免疫过氧化物酶相对于免疫荧光的优势在于它能提供一份永久性标本，数月或数年后仍可复查。此外，由于切片可用梅耶苏木精复染并通过传统光学显微镜观察，它能更准确地定位免疫沉积物。鉴于这些优势，我们建议在检查肾活检材料时，应认真考虑使用免疫过氧化物酶代替免疫荧光法。
