Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, KY 40536-0298, USA.
Brain Restoration Center, College of Medicine, University of Kentucky, Lexington, KY 40536-0298, USA.
Biomolecules. 2023 Mar 25;13(4):594. doi: 10.3390/biom13040594.
The peripheral nervous system (PNS) has a unique ability for self-repair. Dorsal root ganglion (DRG) neurons regulate the expression of different molecules, such as neurotrophins and their receptors, to promote axon regeneration after injury. However, the molecular players driving axonal regrowth need to be better defined. The membrane glycoprotein GPM6a has been described to contribute to neuronal development and structural plasticity in central-nervous-system neurons. Recent evidence indicates that GPM6a interacts with molecules from the PNS, although its role in DRG neurons remains unknown. Here, we characterized the expression of GPM6a in embryonic and adult DRGs by combining analysis of public RNA-seq datasets with immunochemical approaches utilizing cultures of rat DRG explants and dissociated neuronal cells. M6a was detected on the cell surfaces of DRG neurons throughout development. Moreover, GPM6a was required for DRG neurite elongation in vitro. In summary, we provide evidence on GPM6a being present in DRG neurons for the first time. Data from our functional experiments support the idea that GPM6a could contribute to axon regeneration in the PNS.
周围神经系统 (PNS) 具有自我修复的独特能力。背根神经节 (DRG) 神经元调节不同分子的表达，如神经营养因子及其受体，以促进损伤后的轴突再生。然而，驱动轴突再生的分子参与者需要更好地定义。膜糖蛋白 GPM6a 已被描述为有助于中枢神经系统神经元的发育和结构可塑性。最近的证据表明，GPM6a 与 PNS 的分子相互作用，尽管其在 DRG 神经元中的作用仍不清楚。在这里，我们通过结合使用大鼠 DRG 外植体和分离神经元细胞培养物的公共 RNA-seq 数据集分析和免疫化学方法，对 GPM6a 在胚胎和成年 DRG 中的表达进行了表征。M6a 在整个发育过程中都存在于 DRG 神经元的细胞表面。此外，GPM6a 是体外 DRG 神经元突起伸长所必需的。总之，我们首次提供了 GPM6a 存在于 DRG 神经元中的证据。我们的功能实验数据支持 GPM6a 可能有助于 PNS 中的轴突再生的观点。
