Gund Madline P, Naim Jusef, Lehmann Antje, Hannig Matthias, Linsenmann Constanze, Schindler Axel, Rupf Stefan
Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University, 66421 Homburg, Germany.
Leibniz Institute of Surface Modification (IOM), 04318 Leipzig, Germany.
Biomedicines. 2023 Apr 16;11(4):1185. doi: 10.3390/biomedicines11041185.
Cold atmospheric plasma treatment (CAP) enables the contactless modification of titanium. This study aimed to investigate the attachment of primary human gingival fibroblasts on titanium. Machined and microstructured titanium discs were exposed to cold atmospheric plasma, followed by the application of primary human gingival fibroblasts onto the disc. The fibroblast cultures were analyzed by fluorescence, scanning electron microscopy and cell-biological tests. The treated titanium displayed a more homogeneous and denser fibroblast coverage, while its biological behavior was not altered. This study demonstrated for the first time the beneficial effect of CAP treatment on the initial attachment of primary human gingival fibroblasts on titanium. The results support the application of CAP in the context of pre-implantation conditioning, as well as of peri-implant disease treatment.
冷大气等离子体处理（CAP）能够对钛进行非接触式改性。本研究旨在调查原代人牙龈成纤维细胞在钛上的附着情况。将机械加工和微结构化的钛盘暴露于冷大气等离子体，然后将原代人牙龈成纤维细胞接种到盘上。通过荧光、扫描电子显微镜和细胞生物学测试对成纤维细胞培养物进行分析。经处理的钛显示出更均匀、更密集的成纤维细胞覆盖，而其生物学行为未改变。本研究首次证明了CAP处理对原代人牙龈成纤维细胞在钛上的初始附着具有有益作用。这些结果支持了CAP在种植前预处理以及种植周疾病治疗中的应用。
