Jungbauer Gert, Favaro Leandro, Müller Steffen, Sculean Anton, Eick Sigrun
Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland.
Independent Researcher, Private Dental Practice, 94315 Straubing, Germany.
Antibiotics (Basel). 2022 May 31;11(6):752. doi: 10.3390/antibiotics11060752.
Due to its antimicrobial and healing-promoting effects, the application of cold atmospheric plasma (CAP) appears to be a promising modality in various fields of general medicine and dentistry. The aim of the present study was to evaluate the antibacterial and anti-biofilm activity of a handheld device utilizing ambient air for plasma generation. Suspensions of 11 oral bacteria (among them , , , , and ) were exposed to CAP for 10, 30, 60, and 120 s. Before and after treatment, colony forming unit (CFU) counts were determined. Then, 12-species biofilms were cultured on dentin and titanium specimens, and CAP was applied for 30, 60, and 120 s before quantifying CFU counts, biofilm mass, and metabolic activity. A reduction of ≥3 log CFU, was found for ten out of the eleven tested species at 30 s (except for ) and for all species at 60 s. For biofilm grown on dentin and titanium specimens, the log reductions were 2.43 log CFU/specimen and by about 4 log CFU/specimen after 120 s of CAP. The CAP application did not reduce the biomass significantly, the metabolic activity of the biofilms on dentin and titanium decreased by 98% and 95% after 120 s of CAP. An application of 120 s of CAP had no cytotoxic effect on gingival fibroblasts and significantly increased the adhesion of gingival fibroblasts to the titanium surface. These results are promising and underline the potential of CAP for implementation in periodontal and peri-implantitis therapy.
由于其抗菌和促进愈合的作用，冷大气等离子体（CAP）在普通医学和牙科的各个领域似乎是一种很有前景的治疗方式。本研究的目的是评估一种利用环境空气产生等离子体的手持设备的抗菌和抗生物膜活性。将11种口腔细菌（其中包括 、 、 、 、和 ）的悬浮液暴露于CAP中10、30、60和120秒。在处理前后，测定菌落形成单位（CFU）数量。然后，在牙本质和钛标本上培养12种细菌的生物膜，并在量化CFU数量、生物膜质量和代谢活性之前，将CAP应用30、60和120秒。在30秒时，11种受试菌种中有10种（ 除外）的CFU减少≥3 log，在60秒时所有菌种的CFU均减少。对于在牙本质和钛标本上生长的生物膜，在CAP处理120秒后，对数减少分别为2.43 log CFU/标本和约4 log CFU/标本。CAP处理并未显著降低生物量，在CAP处理120秒后，牙本质和钛上生物膜的代谢活性分别下降了98%和95%。120秒的CAP处理对牙龈成纤维细胞没有细胞毒性作用，并且显著增加了牙龈成纤维细胞对钛表面的粘附。这些结果很有前景，并强调了CAP在牙周炎和种植体周围炎治疗中的应用潜力。
