Alharthy Khalid M, Balaha Mohamed F, Devi Sushma, Altharawi Ali, Yusufoglu Hasan S, Aldossari Rana M, Alam Aftab, di Giacomo Viviana
Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
Department of Clinical Pharmacy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
Biomedicines. 2023 Apr 18;11(4):1203. doi: 10.3390/biomedicines11041203.
Diabetic polyneuropathy is characterized by structural abnormalities, oxidative stress, and neuroinflammation. The current study aimed to determine the antinociceptive effects of isoeugenol and eugenol and their combinations in neuropathic pain resulting from streptozotocin (STZ)-induced diabetes and neuroinflammation. Female SD rats were categorized into normal control, diabetic control, and treatment groups. On the 28th day and 45th day, behavioral studies (allodynia and hyperalgesia) were performed to analyze the development and protection of diabetic polyneuropathy. The levels of inflammatory and oxidative mediators, such as superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α), catalase, reduced glutathione, and thiobarbituric acid reactive substances (TBARS), were estimated. In addition, the level of nerve growth factor (NGF) was estimated at the end of the study in different groups. The anti-NGF treatment decreased its upregulation in the dorsal root ganglion significantly. The results showed that isoeugenol, eugenol, and their combination have therapeutic potential against neuronal and oxidative damage induced by diabetes. In particular, both compounds significantly affected behavioral function in treated rats and showed neuroprotection against diabetic neuropathy, and their combination had synergistic effects.
糖尿病性多发性神经病变的特征为结构异常、氧化应激和神经炎症。本研究旨在确定异丁香酚和丁香酚及其组合对链脲佐菌素（STZ）诱导的糖尿病性神经病变性疼痛和神经炎症的抗伤害感受作用。将雌性SD大鼠分为正常对照组、糖尿病对照组和治疗组。在第28天和第45天进行行为学研究（痛觉过敏和痛觉超敏），以分析糖尿病性多发性神经病变的发展和保护情况。测定炎症和氧化介质的水平，如超氧化物歧化酶（SOD）、肿瘤坏死因子-α（TNF-α）、过氧化氢酶、还原型谷胱甘肽和硫代巴比妥酸反应性物质（TBARS）。此外，在研究结束时测定不同组神经生长因子（NGF）的水平。抗NGF治疗显著降低了其在背根神经节中的上调。结果表明，异丁香酚、丁香酚及其组合对糖尿病诱导的神经元和氧化损伤具有治疗潜力。特别是，这两种化合物均显著影响治疗大鼠的行为功能，并显示出对糖尿病性神经病变的神经保护作用，且它们的组合具有协同作用。
