Research Center "E. Piaggio", University of Pisa, Largo Lucio Lazzarino 1, 56122 Pisa, Italy.
Department of Information Engineering (DII), University of Pisa, Via G. Caruso 16, 56122 Pisa, Italy.
Cells. 2023 Apr 18;12(8):1181. doi: 10.3390/cells12081181.
Human-relevant three-dimensional (3D) models of cerebral tissue can be invaluable tools to boost our understanding of the cellular mechanisms underlying brain pathophysiology. Nowadays, the accessibility, isolation and harvesting of human neural cells represents a bottleneck for obtaining reproducible and accurate models and gaining insights in the fields of oncology, neurodegenerative diseases and toxicology. In this scenario, given their low cost, ease of culture and reproducibility, neural cell lines constitute a key tool for developing usable and reliable models of the human brain. Here, we review the most recent advances in 3D constructs laden with neural cell lines, highlighting their advantages and limitations and their possible future applications.
人脑相关的三维（3D）模型可以成为增强我们对大脑病理生理学中细胞机制理解的宝贵工具。如今，人类神经细胞的可及性、分离和采集是获得可重复和准确模型并深入了解肿瘤学、神经退行性疾病和毒理学领域的瓶颈。在这种情况下，由于其成本低、易于培养和可重复性，神经细胞系成为开发人类大脑可用和可靠模型的关键工具。在这里，我们回顾了富含神经细胞系的 3D 构建体的最新进展，强调了它们的优点和局限性及其可能的未来应用。
