Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
Analyst. 2023 May 30;148(11):2609-2615. doi: 10.1039/d3an00646h.
The discovery and implementation of media that derive from bioinspired designs and bear optical readouts featuring large Stokes shifts are of continued interest to a wide variety of researchers and clinicians. Myco-F, a novel mycophenolic acid precursor-based probe features a cleavable -butyldimethylsiloxy group to allow for fluoride detection. Myco-F exhibits high selectivity and specificity towards F (Stokes shift = 120 nm). All measurements were performed in complete aqueous media (LOD=0.38 μM). Myco-F enables detection of fluoride ions in living HEK293 cells and localizes in the eye region (among other regions) of the zebrafish. DFT calculations support the proposed ESIPT working photomechanism.
生物启发设计衍生的并具有大斯托克斯位移光学读出功能的介质的发现和应用一直受到广泛的研究人员和临床医生的关注。Myco-F 是一种新型的基于霉酚酸前体的探针，具有可裂解的丁基二甲基硅氧基，可用于氟离子检测。Myco-F 对 F 具有高选择性和特异性（斯托克斯位移 = 120nm）。所有测量均在完全水介质中进行（LOD=0.38 μM）。Myco-F 可用于检测活 HEK293 细胞中的氟离子，并定位于斑马鱼的眼部区域（以及其他区域）。DFT 计算支持所提出的 ESIPT 工作光机理。
