Oud J L, Reutlinger A H
Chromosoma. 1981;83(3):395-407. doi: 10.1007/BF00327361.
A correlative light- and electron microscopical study has yielded both a description of chromosome behaviour during early meiotic prophase in mouse spermatocytes, and an accurate timing of the zygotene and early pachytene stage. The light microscopic part of the study consists of an analysis of orcein stained or C-banded, air-dried preparations, and series of separately squashed sections of seminiferous tubules. The mice used for air-dried preparations were treated with hyroxyurea and triaziquone, which reduces the spermatocyte population to a small, well-defined group of cells in meiotic interphase. The development of the restricted spermatocyte population is followed during four days. The electron microscopical study consists of an investigation of synaptonemal complexes in thin sections of seminiferous tubules in various stages of the epithelial cycle. It turned out that synaptic pairing starts about half a day after the end of the DNA replication phase. At most one and a half day later chromosome pairing is completed. In very early pachytene spermatocytes a sex vesicle is not visible. The orientation of the X and Y chromosome into a sex vesicle starts about one day after the beginning of the pachytene stage. The existence of a leptotene stage is discussed. The presence of a pre-leptotene contraction phase in male mice is excluded.
一项相关的光学显微镜和电子显微镜研究既描述了小鼠精母细胞减数分裂前期早期的染色体行为，又精确确定了偶线期和粗线期早期的时间。该研究的光学显微镜部分包括对orcein染色或C带染色的风干制片以及一系列单独压片的生精小管切片的分析。用于风干制片的小鼠用羟基脲和三嗪醌处理，这将精母细胞群体减少到处于减数分裂间期的一小群界限清晰的细胞。在四天内追踪受限精母细胞群体的发育情况。电子显微镜研究包括对处于上皮周期不同阶段的生精小管薄片中的联会复合体进行研究。结果表明，突触配对在DNA复制阶段结束后约半天开始。最多再过一天半，染色体配对完成。在极早期粗线期精母细胞中看不到性泡。X和Y染色体向性泡的定向在粗线期开始后约一天开始。讨论了细线期的存在。排除了雄性小鼠中前细线期收缩期的存在。
