Dietrich A J, Mulder R J
Chromosoma. 1981;83(3):409-18. doi: 10.1007/BF00327362.
A method is presented for the sequential analysis of male meiosis using hydroxyurea (HU). HU produces a gap in the spermatogenic line. The front of surviving cells behind the gap was examined day by day using silverstained whole mount spreads on glass slides. With this method it was possible to study the development and behaviour of the synaptonemal complex (SC) in mouse spermatocytes by the light microscope. At zygotene no unpaired axial elements could be seen. Unpaired axial elements were found to be specific for the diplotene stage. The axes of the XY pair could be recognized from late zygotene up to diplotene.
介绍了一种使用羟基脲（HU）对雄性减数分裂进行顺序分析的方法。HU在生精系中产生一个间隙。每天使用载玻片上的银染整装铺片检查间隙后存活细胞的前沿。通过这种方法，可以用光镜研究小鼠精母细胞中联会复合体（SC）的发育和行为。在偶线期看不到未配对的轴向元件。发现未配对的轴向元件是双线期的特异性特征。XY对的轴从偶线期末期到双线期都可以识别。
