Lösecke W, Naumann W, Sterba G
Cell Tissue Res. 1986;244(2):449-56. doi: 10.1007/BF00219221.
Low-temperature-embedded tissue of the subcommissural organ (SCO) of the rabbit was analyzed for the basal route of secretory product by means of indirect immuno-metal cytochemistry (protein A-gold technique) at the electron-microscopic level. By use of (1) an antiserum against bovine Reissner's fibre (see Sterba et al. 1981) and, thereafter, (2) particulate gold-marker solution, immunoreactive sites could be clearly visualized within the extracellular matrix of both (a) the basal part of the ependymal cell layer, and (b) the hypendyma proper. Abundant secretory material was identified within (i) dilated intercellular spaces (a + b) as well as (ii) branching basal lamina labyrinths and distinct perivascular spaces (b). All these compartments are thought to belong to a system of extracellular channels, which may function in secretion directed toward hypendymal blood vessels.
利用间接免疫金属细胞化学法（蛋白A-金技术）在电子显微镜水平上分析了兔连合下器官（SCO）的低温包埋组织，以确定分泌产物的基础途径。通过使用（1）抗牛瑞氏纤维抗血清（见Sterba等人，1981年），然后（2）颗粒状金标记溶液，在（a）室管膜细胞层基部和（b）固有室管膜下层的细胞外基质中都能清晰地看到免疫反应位点。在（i）扩张的细胞间隙（a + b）以及（ii）分支的基膜迷路和明显的血管周围间隙（b）中发现了丰富的分泌物质。所有这些腔室都被认为属于细胞外通道系统，该系统可能在向室管膜下层血管的分泌中发挥作用。
