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在使用Lowicryl 4KM电子显微镜包埋树脂过程中克服的技术难题。

Technical difficulties overcome in the use of Lowicryl 4KM EM embedding resin.

作者信息

Fryer P R, Wells C, Ratcliffe A

出版信息

Histochemistry. 1983;77(1):141-3. doi: 10.1007/BF00496645.

DOI:10.1007/BF00496645
PMID:6188723
Abstract

Several technical difficulties have been overcome in the use of Lowicryl 4KM resin. In order to embed and section tissue satisfactorily in the resin, it has been found necessary to thoroughly degass the resin before infiltration and polymerisation. After irradiation with UV light, the blocks are further polymerised by exposure to daylight for 2-3 weeks and then stored under partial vacuum over dessicant.

摘要

在使用Lowicryl 4KM树脂的过程中,已经克服了一些技术难题。为了在该树脂中令人满意地包埋和切片组织,已发现有必要在渗透和聚合之前对树脂进行彻底脱气。用紫外线照射后,将包埋块暴露在日光下2至3周以进一步聚合,然后在干燥剂上于部分真空下储存。

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本文引用的文献

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J Histochem Cytochem. 1982 Feb;30(2):139-47. doi: 10.1177/30.2.7061817.
2
Immunocytochemical localization of kallikrein in the rat exocrine pancreas.大鼠外分泌胰腺中激肽释放酶的免疫细胞化学定位
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3
Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.低温包埋胰腺组织中结构保存和免疫细胞化学染色的增强
软骨细胞中的蛋白聚糖生物合成:高尔基亚区室中蛋白聚糖蛋白核心和硫酸软骨素的蛋白A-金定位
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Immuno-electron-microscopic analysis of the basal route of secretion in the subcommissural organ of the rabbit.兔连合下器官分泌基础途径的免疫电子显微镜分析
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Evidence that major 78-44-kD concanavalin A-binding glycopolypeptides in pig epidermis arise from the degradation of desmosomal glycoproteins during terminal differentiation.有证据表明,猪表皮中主要的78 - 44kD伴刀豆球蛋白A结合糖多肽是在终末分化过程中桥粒糖蛋白降解产生的。
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Immuno-electron microscopy of chondrocyte-derived cells in the rheumatoid cartilage-pannus junction.类风湿性关节炎软骨-血管翳交界处软骨细胞衍生细胞的免疫电子显微镜检查。
Rheumatol Int. 1988;8(5):231-4. doi: 10.1007/BF00269200.
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Intracellular localization of rotaviral proteins.
Arch Virol. 1986;88(3-4):251-64. doi: 10.1007/BF01310879.
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