Gai Qing-Yan, Feng Xue, Jiao Jiao, Xu Xiao-Jie, Fu Jin-Xian, He Xiao-Jia, Fu Yu-Jie
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040 People's Republic of China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040 People's Republic of China.
Plant Cell Tissue Organ Cult. 2023;153(3):511-523. doi: 10.1007/s11240-023-02486-7. Epub 2023 Mar 28.
The root of s (Fisch.) Bunge is one of the most frequently used herbs in traditional Chinese medicine (TCM) formulae for fighting COVID-19 infections, due to the presence of isoflavonoids and astragalosides associated with antiviral and immune-enhancing activities. For the first time, the exposure of hairy root cultures (AMHRCs) to different colors of LED lights i.e., red, green, blue, red/green/blue (1/1/1, RGB), and white, was conducted to promote the root growth and accumulation of isoflavonoids and astragalosides. LED light treatment regardless of colors was found beneficial for root growth, which might be a result of the formation of more root hairs upon light stimulation. Blue LED light was found most effective for enhancing phytochemical accumulation. Results showed that the productivity of root biomass in blue-light grown AMHRCs with an initial inoculum size of 0.6% for 55 days was 1.40-fold higher than that in dark (control), and yields of high-value isoflavonoids and astragalosides including calycosin, formononetin, astragaloside IV, and astragaloside I increased by 3.17-fold, 2.66-fold, 1.78-fold, and 1.52-fold relative to control, respectively. Moreover, the photooxidative stress together with transcriptional activation of biosynthesis genes might contribute to the enhanced accumulation of isoflavonoids and astragalosides in blue-light grown AMHRCs. Overall, this work offered a feasible approach for obtaining higher yields of root biomass and medicinally important compounds in AMHRCs via the simple supplementation of blue LED light, which made blue-light grown AMHRCs industrially attractive as plant factory in controlled growing systems.
The online version contains supplementary material available at 10.1007/s11240-023-02486-7.
蒙古黄芪（Fisch.）Bunge的根是用于抗击新冠病毒感染的中药配方中最常用的草药之一，这是因为其含有具有抗病毒和免疫增强活性的异黄酮类化合物和黄芪皂苷。首次对毛状根培养物（AMHRCs）进行不同颜色LED光（即红色、绿色、蓝色、红/绿/蓝（1/1/1，RGB）和白色）照射，以促进根的生长以及异黄酮类化合物和黄芪皂苷的积累。结果发现，无论何种颜色，LED光处理都有利于根的生长，这可能是光刺激后形成更多根毛的结果。发现蓝色LED光对增强植物化学物质积累最有效。结果表明，初始接种量为0.6%的AMHRCs在蓝光下培养55天，其根生物量的生产率比黑暗（对照）条件下高1.40倍，包括毛蕊异黄酮、芒柄花素、黄芪甲苷IV和黄芪甲苷I在内的高价值异黄酮类化合物和黄芪皂苷的产量分别比对照增加了3.17倍、2.66倍、1.78倍和1.52倍。此外，光氧化应激以及生物合成基因的转录激活可能有助于蓝光培养的AMHRCs中异黄酮类化合物和黄芪皂苷积累的增强。总体而言，这项工作提供了一种可行的方法，即通过简单补充蓝色LED光，在AMHRCs中获得更高产量的根生物量和具有药用价值的重要化合物，这使得蓝光培养的AMHRCs在可控生长系统中作为植物工厂在工业上具有吸引力。
在线版本包含可在10.1007/s11240-023-02486-7获取的补充材料。
