Savettieri G, Cestelli A, Ferraro D, Salemi G, Castiglia D, Di Liegro I
Cell Biol Int Rep. 1986 May;10(5):339-45. doi: 10.1016/0309-1651(86)90005-6.
Neurons from rat fetal cerebral hemispheres were grown in a synthetic medium (Maat medium), as previously described, for different periods of time. The repeat length of their chromatin was determined by micrococcal nuclease digestion and compared with that of neurons isolated from postnatal rat brain of corresponding ages. In contrast to the in vivo situation, we found that neurons, dissociated at the 16th gestational day and cultured in vitro, did not undergo the shortening of their chromatin repeat, thus indicating that, at the end of their mitotic cycles, they are not yet programmed to this event.
如前所述，将来自大鼠胎儿脑半球的神经元在合成培养基（Maat培养基）中培养不同时间段。通过微球菌核酸酶消化测定其染色质的重复长度，并与从相应年龄的新生大鼠脑中分离的神经元的染色质重复长度进行比较。与体内情况相反，我们发现，在妊娠第16天解离并在体外培养的神经元并未经历染色质重复长度的缩短，因此表明，在其有丝分裂周期结束时，它们尚未被编程进行这一过程。
